Project name: SH3_W119N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119N
Energy difference between WT (input) and mutated protein (by FoldX)	1.92577 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.2276
• Maximal score value: 1.7964
• Average score: -1.1607
• Total score value: -66.161

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9232
88	V	A	0.4303
89	A	A	0.0000
90	L	A	-0.1542
91	Y	A	-0.5799
92	D	A	-2.5689
93	Y	A	-1.9795
94	E	A	-2.6895
95	A	A	-2.7152
96	R	A	-3.0461
97	T	A	-2.7305
98	E	A	-3.1756
99	D	A	-3.1638
100	D	A	-2.5408
101	L	A	0.0000
102	S	A	-2.2676
103	F	A	0.0000
104	H	A	-2.7333
105	K	A	-2.4098
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0562
112	L	A	0.1436
113	N	A	-1.1136
114	S	A	-1.5157
115	S	A	-1.8020
116	E	A	-2.8677
117	G	A	-2.6032
118	D	A	-3.2276
119	N	A	-2.7352	mutated: WA119N
120	W	A	-1.8824
121	E	A	-1.5628
122	A	A	0.0000
123	R	A	-1.7147
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7273
131	G	A	-1.7715
132	Y	A	-1.4527
133	I	A	0.0000
134	P	A	-1.2602
135	S	A	-1.4480
136	N	A	-1.3986
137	Y	A	-0.3065
138	V	A	0.0000
139	A	A	0.4154
140	P	A	0.7757
141	V	A	1.7964