Project name: SH3_V88N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88N
Energy difference between WT (input) and mutated protein (by FoldX)	2.29909 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.5968
• Average score: -1.0183
• Total score value: -58.0433

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4454
86	L	A	0.6361
87	F	A	0.5794
88	N	A	-0.2688	mutated: VA88N
89	A	A	0.0000
90	L	A	-0.3568
91	Y	A	-0.6758
92	D	A	-2.5662
93	Y	A	-1.9353
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.8119
105	K	A	-2.5476
106	G	A	-1.6805
107	E	A	-1.5217
108	K	A	-0.9341
109	F	A	0.0000
110	Q	A	-0.5912
111	I	A	-0.0574
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7052
124	S	A	0.0000
125	L	A	0.0243
126	T	A	-0.4524
127	T	A	-0.8087
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1549
137	Y	A	-0.2415
138	V	A	0.0000
139	A	A	0.1837
140	P	A	0.5933
141	V	A	1.5968