Project name: SH3_L112D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112D
Energy difference between WT (input) and mutated protein (by FoldX)	1.66633 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1026
• Maximal score value: 1.7964
• Average score: -1.1776
• Total score value: -67.1213

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4896
86	L	A	0.6861
87	F	A	0.8758
88	V	A	0.3961
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6253
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1026
99	D	A	-3.0435
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2041
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3372
108	K	A	-0.7749
109	F	A	0.0000
110	Q	A	-1.4458
111	I	A	-1.4868
112	D	A	-2.5720	mutated: LA112D
113	N	A	-2.2727
114	S	A	-1.8129
115	S	A	-1.9388
116	E	A	-2.5898
117	G	A	-2.1499
118	D	A	-2.4563
119	W	A	-1.1181
120	W	A	-1.6600
121	E	A	-2.1303
122	A	A	0.0000
123	R	A	-2.6734
124	S	A	0.0000
125	L	A	-0.2007
126	T	A	-0.5878
127	T	A	-0.9060
128	G	A	-1.5028
129	E	A	-2.3632
130	T	A	-2.2181
131	G	A	-1.8679
132	Y	A	-1.2097
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9170
136	N	A	-1.1501
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7600
141	V	A	1.7964