Project name: SH3_L112N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112N
Energy difference between WT (input) and mutated protein (by FoldX)	1.11679 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -1.0945
• Total score value: -62.3891

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4722
86	L	A	0.7254
87	F	A	0.8609
88	V	A	0.3896
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0295
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2055
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3252
108	K	A	-0.6826
109	F	A	0.0000
110	Q	A	-1.1872
111	I	A	-1.0587
112	N	A	-1.8678	mutated: LA112N
113	N	A	-1.9027
114	S	A	-1.6267
115	S	A	-1.8397
116	E	A	-2.5554
117	G	A	-2.1284
118	D	A	-2.4359
119	W	A	-1.0807
120	W	A	-1.4132
121	E	A	-1.6955
122	A	A	0.0000
123	R	A	-1.9932
124	S	A	0.0000
125	L	A	-0.0646
126	T	A	-0.5232
127	T	A	-0.8838
128	G	A	-1.4942
129	E	A	-2.2516
130	T	A	-1.8957
131	G	A	-1.6448
132	Y	A	-1.0084
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9017
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7500
141	V	A	1.7964