Project name: SH3_D92L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	DA92L
Energy difference between WT (input) and mutated protein (by FoldX)	1.06641 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1026
• Maximal score value: 1.7964
• Average score: -0.7271
• Total score value: -41.446

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9239
88	V	A	0.4314
89	A	A	0.0000
90	L	A	0.5306
91	Y	A	1.0505
92	L	A	0.7773	mutated: DA92L
93	Y	A	-0.2967
94	E	A	-1.8880
95	A	A	-2.2838
96	R	A	-2.9848
97	T	A	-2.6606
98	E	A	-3.1026
99	D	A	-3.0432
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-1.6010
103	F	A	0.0000
104	H	A	-1.2189
105	K	A	-1.1035
106	G	A	-0.9479
107	E	A	-0.8518
108	K	A	-0.6418
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0596
126	T	A	-0.4724
127	T	A	-0.8204
128	G	A	-1.3572
129	E	A	-2.2400
130	T	A	-1.6926
131	G	A	-1.4990
132	Y	A	-0.8672
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	0.3794
138	V	A	0.0000
139	A	A	0.4153
140	P	A	0.7757
141	V	A	1.7964