Project name: SH3_L112P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112P
Energy difference between WT (input) and mutated protein (by FoldX)	5.4718 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.7964
• Average score: -1.0525
• Total score value: -59.9904

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4969
86	L	A	0.7270
87	F	A	0.8892
88	V	A	0.4039
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6256
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1029
99	D	A	-3.0560
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2045
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3275
108	K	A	-0.7191
109	F	A	0.0000
110	Q	A	-0.9394
111	I	A	-0.6201
112	P	A	-0.9024	mutated: LA112P
113	N	A	-1.4031
114	S	A	-1.4145
115	S	A	-1.7245
116	E	A	-2.5746
117	G	A	-2.1450
118	D	A	-2.4562
119	W	A	-1.1269
120	W	A	-1.3218
121	E	A	-1.6115
122	A	A	0.0000
123	R	A	-2.1236
124	S	A	0.0000
125	L	A	-0.0890
126	T	A	-0.5299
127	T	A	-0.8520
128	G	A	-1.4077
129	E	A	-2.2787
130	T	A	-1.9209
131	G	A	-1.6693
132	Y	A	-1.0323
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9288
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7622
141	V	A	1.7964