Project name: SH3_E107T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107T
Energy difference between WT (input) and mutated protein (by FoldX)	0.930995 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1047
• Maximal score value: 1.8005
• Average score: -0.9258
• Total score value: -52.772

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7949
87	F	A	0.9619
88	V	A	0.5079
89	A	A	0.0000
90	L	A	-0.1154
91	Y	A	-0.5465
92	D	A	-2.5323
93	Y	A	-1.9365
94	E	A	-2.6515
95	A	A	-2.6320
96	R	A	-2.9876
97	T	A	-2.6626
98	E	A	-3.1047
99	D	A	-3.0450
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2201
103	F	A	0.0000
104	H	A	-2.6602
105	K	A	-2.3330
106	G	A	-1.3440
107	T	A	0.0000	mutated: EA107T
108	K	A	-0.5214
109	F	A	0.0000
110	Q	A	-0.5112
111	I	A	-0.0542
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7175
124	S	A	0.0000
125	L	A	0.0805
126	T	A	-0.4287
127	T	A	-0.7994
128	G	A	-1.3630
129	E	A	-2.2379
130	T	A	-1.6934
131	G	A	-1.4992
132	Y	A	-0.8693
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4187
140	P	A	0.7786
141	V	A	1.8005