Project name: SH3_Y93A

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93A
Energy difference between WT (input) and mutated protein (by FoldX)	4.71544 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1595
• Maximal score value: 1.7964
• Average score: -1.0334
• Total score value: -58.9041

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9290
88	V	A	0.4376
89	A	A	0.0000
90	L	A	-0.1587
91	Y	A	-0.6040
92	D	A	-2.6297
93	A	A	-2.1032	mutated: YA93A
94	E	A	-2.7486
95	A	A	-2.7308
96	R	A	-3.0477
97	T	A	-2.7139
98	E	A	-3.1595
99	D	A	-3.1380
100	D	A	-2.2792
101	L	A	0.0000
102	S	A	-2.3006
103	F	A	0.0000
104	H	A	-2.7691
105	K	A	-2.4283
106	G	A	-1.4605
107	E	A	-1.3109
108	K	A	-0.6443
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0573
112	L	A	0.1425
113	N	A	-0.8930
114	S	A	-1.1865
115	S	A	-1.6003
116	E	A	-2.5660
117	G	A	-2.1458
118	D	A	-2.4665
119	W	A	-1.1669
120	W	A	-1.1013
121	E	A	-1.1928
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7211
131	G	A	-1.5713
132	Y	A	-0.9682
133	I	A	0.0000
134	P	A	-0.6254
135	S	A	-0.9260
136	N	A	-1.1379
137	Y	A	-0.1102
138	V	A	0.0000
139	A	A	0.4261
140	P	A	0.7757
141	V	A	1.7964