Project name: SH3_Y132L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132L
Energy difference between WT (input) and mutated protein (by FoldX)	0.314241 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0946
• Maximal score value: 1.7964
• Average score: -0.9803
• Total score value: -55.8752

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6172
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.0946
99	D	A	-3.0661
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1973
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5499
111	I	A	-0.1276
112	L	A	0.0615
113	N	A	-0.9567
114	S	A	-1.2259
115	S	A	-1.6197
116	E	A	-2.5928
117	G	A	-2.1568
118	D	A	-2.4618
119	W	A	-1.1402
120	W	A	-1.1823
121	E	A	-1.4527
122	A	A	0.0000
123	R	A	-1.7770
124	S	A	0.0000
125	L	A	0.0591
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3571
129	E	A	-2.2399
130	T	A	-1.7556
131	G	A	-1.5782
132	L	A	-0.9360	mutated: YA132L
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9488
136	N	A	-1.1478
137	Y	A	-0.1225
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964