Project name: SH3_S135A

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135A
Energy difference between WT (input) and mutated protein (by FoldX)	-0.140501 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9573
• Total score value: -54.564

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9287
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1506
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5090
111	I	A	-0.0526
112	L	A	0.1431
113	N	A	-0.8876
114	S	A	-1.1729
115	S	A	-1.5961
116	E	A	-2.5597
117	G	A	-2.1288
118	D	A	-2.4322
119	W	A	-1.0899
120	W	A	-1.0441
121	E	A	-1.1479
122	A	A	0.0000
123	R	A	-1.7154
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4990
132	Y	A	-0.8619
133	I	A	0.0000
134	P	A	0.0000
135	A	A	-0.8820	mutated: SA135A
136	N	A	-1.1313
137	Y	A	-0.1103
138	V	A	0.0000
139	A	A	0.4225
140	P	A	0.7757
141	V	A	1.7964