Project name: SH3_L112C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112C
Energy difference between WT (input) and mutated protein (by FoldX)	2.21784 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.7964
• Average score: -1.014
• Total score value: -57.7956

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4696
86	L	A	0.6724
87	F	A	0.8519
88	V	A	0.3833
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6256
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1029
99	D	A	-3.0537
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2041
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3372
108	K	A	-0.7644
109	F	A	0.0000
110	Q	A	-0.8831
111	I	A	-0.2999
112	C	A	-0.1313	mutated: LA112C
113	N	A	-1.0214
114	S	A	-1.2309
115	S	A	-1.6259
116	E	A	-2.5720
117	G	A	-2.1430
118	D	A	-2.4540
119	W	A	-1.1229
120	W	A	-1.1686
121	E	A	-1.3540
122	A	A	0.0000
123	R	A	-2.0363
124	S	A	0.0000
125	L	A	-0.1682
126	T	A	-0.5580
127	T	A	-0.8719
128	G	A	-1.4479
129	E	A	-2.2948
130	T	A	-1.8410
131	G	A	-1.5785
132	Y	A	-0.9413
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9282
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7500
141	V	A	1.7964