Project name: SH3_L90N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA90N
Energy difference between WT (input) and mutated protein (by FoldX)	1.63506 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8006
• Average score: -1.0315
• Total score value: -58.795

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5193
86	L	A	0.7954
87	F	A	0.8029
88	V	A	0.1766
89	A	A	0.0000
90	N	A	-1.2177	mutated: LA90N
91	Y	A	-1.0892
92	D	A	-2.7792
93	Y	A	-2.0625
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.8907
105	K	A	-2.7712
106	G	A	-1.7245
107	E	A	-1.4659
108	K	A	-0.7554
109	F	A	0.0000
110	Q	A	-0.5087
111	I	A	-0.0577
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7167
124	S	A	0.0000
125	L	A	0.0583
126	T	A	-0.4748
127	T	A	-0.8215
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9200
136	N	A	-1.3017
137	Y	A	-0.4599
138	V	A	0.0000
139	A	A	0.1848
140	P	A	0.7805
141	V	A	1.8006