Project name: SH3_V88Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88Y
Energy difference between WT (input) and mutated protein (by FoldX)	2.169 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.822
• Average score: -0.9605
• Total score value: -54.746

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5254
86	L	A	0.8174
87	F	A	0.9537
88	Y	A	0.4900	mutated: VA88Y
89	A	A	0.0000
90	L	A	-0.2895
91	Y	A	-0.6407
92	D	A	-2.5930
93	Y	A	-1.9513
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7418
105	K	A	-2.4431
106	G	A	-1.4694
107	E	A	-1.2929
108	K	A	-0.6055
109	F	A	0.0000
110	Q	A	-0.4887
111	I	A	-0.0574
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7052
124	S	A	0.0000
125	L	A	0.1109
126	T	A	-0.4524
127	T	A	-0.8087
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1743
137	Y	A	-0.1616
138	V	A	0.0000
139	A	A	0.4055
140	P	A	0.7977
141	V	A	1.8220