Project name: SH3_T97P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA97P
Energy difference between WT (input) and mutated protein (by FoldX)	3.06832 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1437
• Maximal score value: 1.7964
• Average score: -0.9645
• Total score value: -54.9754

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9235
88	V	A	0.4306
89	A	A	0.0000
90	L	A	-0.1515
91	Y	A	-0.5751
92	D	A	-2.5603
93	Y	A	-1.9316
94	E	A	-2.6551
95	A	A	-2.6505
96	R	A	-3.0249
97	P	A	-2.7430	mutated: TA97P
98	E	A	-3.1437
99	D	A	-3.0718
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2167
103	F	A	0.0000
104	H	A	-2.7290
105	K	A	-2.4066
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5090
111	I	A	-0.0572
112	L	A	0.1425
113	N	A	-0.8884
114	S	A	-1.1799
115	S	A	-1.5968
116	E	A	-2.5608
117	G	A	-2.1363
118	D	A	-2.4451
119	W	A	-1.1024
120	W	A	-1.0560
121	E	A	-1.1546
122	A	A	0.0000
123	R	A	-1.7162
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6939
131	G	A	-1.5016
132	Y	A	-0.8719
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9204
136	N	A	-1.1498
137	Y	A	-0.1240
138	V	A	0.0000
139	A	A	0.4159
140	P	A	0.7757
141	V	A	1.7964