| Chain sequence(s) |
A: RSFRGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMWT |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | SA21A |
| Energy difference between WT (input) and mutated protein (by FoldX) | 0.459078 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 20 | R | A | -1.7132 | |
| 21 | A | A | 0.0000 | mutated: SA21A |
| 22 | F | A | 1.0772 | |
| 23 | R | A | -0.1278 | |
| 24 | G | A | -0.2013 | |
| 25 | P | A | -0.5244 | |
| 26 | L | A | 0.0000 | |
| 27 | L | A | -0.5618 | |
| 28 | P | A | -0.9189 | |
| 29 | N | A | -1.7163 | |
| 30 | R | A | -1.1600 | |
| 31 | P | A | 0.0000 | |
| 32 | F | A | 0.0000 | |
| 33 | T | A | 0.0000 | |
| 34 | T | A | 0.0000 | |
| 35 | V | A | 0.0000 | |
| 36 | W | A | 0.0000 | |
| 37 | N | A | 0.0000 | |
| 38 | A | A | 0.0000 | |
| 39 | N | A | -0.3043 | |
| 40 | T | A | 0.0000 | |
| 41 | Q | A | -1.3812 | |
| 42 | W | A | -1.8047 | |
| 43 | C | A | 0.0000 | |
| 44 | L | A | -1.7980 | |
| 45 | E | A | -2.7879 | |
| 46 | R | A | -3.1663 | |
| 47 | H | A | -2.8388 | |
| 48 | G | A | -2.0235 | |
| 49 | V | A | 0.0000 | |
| 50 | D | A | -2.2822 | |
| 51 | V | A | 0.0000 | |
| 52 | D | A | -1.4944 | |
| 53 | V | A | 0.0064 | |
| 54 | S | A | 0.0053 | |
| 55 | V | A | 0.2727 | |
| 56 | F | A | 0.0000 | |
| 57 | D | A | -0.1806 | |
| 58 | V | A | 0.0000 | |
| 59 | V | A | 1.1606 | |
| 60 | A | A | 0.2473 | |
| 61 | N | A | -0.1800 | |
| 62 | P | A | -0.0072 | |
| 63 | G | A | -0.5680 | |
| 64 | Q | A | -0.7800 | |
| 65 | T | A | -0.1269 | |
| 66 | F | A | 0.6744 | |
| 67 | R | A | -1.2973 | |
| 68 | G | A | -0.7897 | |
| 69 | P | A | -1.1115 | |
| 70 | D | A | -1.0090 | |
| 71 | M | A | -0.5389 | |
| 72 | T | A | -0.4057 | |
| 73 | I | A | 0.0000 | |
| 74 | F | A | 0.0000 | |
| 75 | Y | A | -0.1029 | |
| 76 | S | A | -0.4527 | |
| 77 | S | A | -0.3792 | |
| 78 | Q | A | -0.7595 | |
| 79 | L | A | 0.0000 | |
| 80 | G | A | -0.6724 | |
| 81 | T | A | 0.0200 | |
| 82 | Y | A | 0.0000 | |
| 83 | P | A | 0.0000 | |
| 84 | Y | A | 0.2277 | |
| 85 | Y | A | 0.0000 | |
| 86 | T | A | -0.6763 | |
| 87 | P | A | -0.6065 | |
| 88 | T | A | -0.7488 | |
| 89 | G | A | -1.0067 | |
| 90 | E | A | -1.6947 | |
| 91 | P | A | -0.5784 | |
| 92 | V | A | 0.7567 | |
| 93 | F | A | 1.4175 | |
| 94 | G | A | 0.1674 | |
| 95 | G | A | 0.0000 | |
| 96 | L | A | 0.0000 | |
| 97 | P | A | 0.0000 | |
| 98 | Q | A | -1.1605 | |
| 99 | N | A | -1.0940 | |
| 100 | A | A | 0.1363 | |
| 101 | S | A | 0.6454 | |
| 102 | L | A | 0.9671 | |
| 103 | I | A | 2.0146 | |
| 104 | A | A | 0.7208 | |
| 105 | H | A | 0.0000 | |
| 106 | L | A | 0.6420 | |
| 107 | A | A | -0.0883 | |
| 108 | R | A | -1.5944 | |
| 109 | T | A | 0.0000 | |
| 110 | F | A | 0.0456 | |
| 111 | Q | A | -1.0169 | |
| 112 | D | A | -0.7650 | |
| 113 | I | A | 0.0000 | |
| 114 | L | A | 0.4261 | |
| 115 | A | A | -0.0734 | |
| 116 | A | A | -0.4558 | |
| 117 | I | A | 0.0000 | |
| 118 | P | A | -0.6060 | |
| 119 | A | A | -0.7159 | |
| 120 | P | A | -1.3614 | |
| 121 | D | A | -2.5213 | |
| 122 | F | A | 0.0000 | |
| 123 | S | A | -1.6077 | |
| 124 | G | A | 0.0000 | |
| 125 | L | A | 0.0000 | |
| 126 | A | A | 0.0000 | |
| 127 | V | A | 0.0000 | |
| 128 | I | A | 0.0000 | |
| 129 | D | A | -0.7110 | |
| 130 | W | A | 0.0000 | |
| 131 | E | A | -1.9042 | |
| 132 | A | A | -0.9617 | |
| 133 | W | A | 0.0000 | |
| 134 | R | A | -0.8754 | |
| 135 | P | A | 0.0000 | |
| 136 | R | A | -0.8423 | |
| 137 | W | A | -0.5947 | |
| 138 | A | A | -0.1950 | |
| 139 | F | A | 0.0000 | |
| 140 | N | A | 0.0000 | |
| 141 | W | A | -0.7590 | |
| 142 | D | A | -1.7951 | |
| 143 | T | A | -1.0457 | |
| 144 | K | A | -1.2403 | |
| 145 | D | A | -1.2474 | |
| 146 | I | A | -0.8999 | |
| 147 | Y | A | 0.0000 | |
| 148 | R | A | -1.5658 | |
| 149 | Q | A | -1.8478 | |
| 150 | R | A | -1.4769 | |
| 151 | S | A | 0.0000 | |
| 152 | R | A | -2.1893 | |
| 153 | A | A | -1.3314 | |
| 154 | L | A | -0.9302 | |
| 155 | V | A | 0.0000 | |
| 156 | Q | A | -1.7426 | |
| 157 | A | A | -1.1390 | |
| 158 | Q | A | -1.6262 | |
| 159 | H | A | -1.5994 | |
| 160 | P | A | -1.6469 | |
| 161 | D | A | -2.1301 | |
| 162 | W | A | -1.2159 | |
| 163 | P | A | -1.0327 | |
| 164 | A | A | -1.0864 | |
| 165 | P | A | -1.1317 | |
| 166 | Q | A | -1.3287 | |
| 167 | V | A | 0.0000 | |
| 168 | E | A | -2.7009 | |
| 169 | A | A | -1.6972 | |
| 170 | V | A | -1.5312 | |
| 171 | A | A | 0.0000 | |
| 172 | Q | A | -2.3148 | |
| 173 | D | A | -2.6437 | |
| 174 | Q | A | -2.3692 | |
| 175 | F | A | 0.0000 | |
| 176 | Q | A | -1.7834 | |
| 177 | G | A | -1.8172 | |
| 178 | A | A | -1.4329 | |
| 179 | A | A | 0.0000 | |
| 180 | R | A | -1.8008 | |
| 181 | A | A | -0.8128 | |
| 182 | W | A | 0.0000 | |
| 183 | M | A | 0.0000 | |
| 184 | A | A | -0.7572 | |
| 185 | G | A | -0.5715 | |
| 186 | T | A | 0.0000 | |
| 187 | L | A | 0.0000 | |
| 188 | Q | A | -1.0483 | |
| 189 | L | A | -0.1087 | |
| 190 | G | A | 0.0000 | |
| 191 | R | A | -1.0240 | |
| 192 | A | A | -0.3755 | |
| 193 | L | A | -0.2530 | |
| 194 | R | A | 0.0000 | |
| 195 | P | A | -1.7326 | |
| 196 | R | A | -2.6511 | |
| 197 | G | A | 0.0000 | |
| 198 | L | A | 0.0000 | |
| 199 | W | A | 0.0000 | |
| 200 | G | A | 0.0000 | |
| 201 | F | A | 0.0000 | |
| 202 | Y | A | 0.0000 | |
| 203 | G | A | -0.4646 | |
| 204 | F | A | 0.0000 | |
| 205 | P | A | 0.0000 | |
| 206 | D | A | -0.3521 | |
| 207 | C | A | 0.0000 | |
| 208 | Y | A | 0.0000 | |
| 209 | N | A | 0.0000 | |
| 210 | Y | A | 0.0971 | |
| 211 | D | A | -1.3121 | |
| 212 | F | A | 0.0000 | |
| 213 | L | A | 1.0472 | |
| 214 | S | A | -0.2393 | |
| 215 | P | A | -0.7333 | |
| 216 | N | A | -1.3749 | |
| 217 | Y | A | -0.8572 | |
| 218 | T | A | -1.0013 | |
| 219 | G | A | 0.0000 | |
| 220 | Q | A | -1.4971 | |
| 221 | C | A | 0.0000 | |
| 222 | P | A | -0.8915 | |
| 223 | S | A | -0.7184 | |
| 224 | G | A | -0.8348 | |
| 225 | I | A | 0.0000 | |
| 226 | R | A | -1.8332 | |
| 227 | A | A | -1.4467 | |
| 228 | Q | A | -1.5558 | |
| 229 | N | A | 0.0000 | |
| 230 | D | A | -2.5556 | |
| 231 | Q | A | -2.1078 | |
| 232 | L | A | 0.0000 | |
| 233 | G | A | -1.0598 | |
| 234 | W | A | -0.6442 | |
| 235 | L | A | 0.0000 | |
| 236 | W | A | 0.0000 | |
| 237 | G | A | -0.6572 | |
| 238 | Q | A | -0.5643 | |
| 239 | S | A | 0.0000 | |
| 240 | R | A | -0.9273 | |
| 241 | A | A | 0.0000 | |
| 242 | L | A | 0.0000 | |
| 243 | Y | A | 0.0000 | |
| 244 | P | A | 0.0000 | |
| 245 | S | A | -0.0457 | |
| 246 | I | A | 0.0000 | |
| 247 | Y | A | 0.0424 | |
| 248 | M | A | 0.0000 | |
| 249 | P | A | 0.3034 | |
| 250 | A | A | 0.0958 | |
| 251 | V | A | 1.4800 | |
| 252 | L | A | 0.0000 | |
| 253 | E | A | -1.1034 | |
| 254 | G | A | -0.9363 | |
| 255 | T | A | -0.6720 | |
| 256 | G | A | -0.9589 | |
| 257 | K | A | -0.6372 | |
| 258 | S | A | 0.0000 | |
| 259 | Q | A | -0.9222 | |
| 260 | M | A | -0.5461 | |
| 261 | Y | A | 0.0000 | |
| 262 | V | A | 0.0000 | |
| 263 | Q | A | -0.7318 | |
| 264 | H | A | -0.6583 | |
| 265 | R | A | 0.0000 | |
| 266 | V | A | 0.0000 | |
| 267 | A | A | -0.3454 | |
| 268 | E | A | 0.0000 | |
| 269 | A | A | 0.0000 | |
| 270 | F | A | 0.2839 | |
| 271 | R | A | -0.2102 | |
| 272 | V | A | 0.0000 | |
| 273 | A | A | 0.0000 | |
| 274 | V | A | 1.3428 | |
| 275 | A | A | 0.2954 | |
| 276 | A | A | -0.2293 | |
| 277 | G | A | -0.3808 | |
| 278 | D | A | 0.0000 | |
| 279 | P | A | -0.1971 | |
| 280 | N | A | -0.4980 | |
| 281 | L | A | 0.0000 | |
| 282 | P | A | 0.0000 | |
| 283 | V | A | 0.0000 | |
| 284 | L | A | 0.0000 | |
| 285 | P | A | 0.0000 | |
| 286 | Y | A | 0.0000 | |
| 287 | V | A | 0.0000 | |
| 288 | Q | A | 0.0000 | |
| 289 | I | A | 0.0000 | |
| 290 | F | A | 0.0000 | |
| 291 | Y | A | 0.0000 | |
| 292 | D | A | -0.5475 | |
| 293 | T | A | -0.8301 | |
| 294 | T | A | -1.1354 | |
| 295 | N | A | -1.6931 | |
| 296 | H | A | -1.4539 | |
| 297 | F | A | -0.2843 | |
| 298 | L | A | 0.0000 | |
| 299 | P | A | -0.3239 | |
| 300 | L | A | 0.4691 | |
| 301 | D | A | -1.3370 | |
| 302 | E | A | 0.0000 | |
| 303 | L | A | 0.0000 | |
| 304 | E | A | -0.8249 | |
| 305 | H | A | -1.0637 | |
| 306 | S | A | 0.0000 | |
| 307 | L | A | 0.0000 | |
| 308 | G | A | 0.0000 | |
| 309 | E | A | 0.0000 | |
| 310 | S | A | 0.0000 | |
| 311 | A | A | 0.0000 | |
| 312 | A | A | 0.0000 | |
| 313 | Q | A | -0.3548 | |
| 314 | G | A | 0.0000 | |
| 315 | A | A | 0.0000 | |
| 316 | A | A | 0.0000 | |
| 317 | G | A | 0.0000 | |
| 318 | V | A | 0.0000 | |
| 319 | V | A | 0.0000 | |
| 320 | L | A | 0.0000 | |
| 321 | W | A | 0.4125 | |
| 322 | V | A | 0.0000 | |
| 323 | S | A | 0.0000 | |
| 324 | W | A | -0.4844 | |
| 325 | E | A | -2.0965 | |
| 326 | N | A | 0.0000 | |
| 327 | T | A | 0.0000 | |
| 328 | R | A | -2.9694 | |
| 329 | T | A | -2.7963 | |
| 330 | K | A | -3.3130 | |
| 331 | E | A | -3.1656 | |
| 332 | S | A | -2.5194 | |
| 333 | C | A | 0.0000 | |
| 334 | Q | A | -2.6713 | |
| 335 | A | A | -1.9539 | |
| 336 | I | A | 0.0000 | |
| 337 | K | A | -2.6423 | |
| 338 | E | A | -2.6691 | |
| 339 | Y | A | -1.3358 | |
| 340 | M | A | 0.0000 | |
| 341 | D | A | -1.8507 | |
| 342 | T | A | -0.8560 | |
| 343 | T | A | -0.1965 | |
| 344 | L | A | 0.0000 | |
| 345 | G | A | 0.0000 | |
| 346 | P | A | -0.0913 | |
| 347 | F | A | 0.0000 | |
| 348 | I | A | 0.0000 | |
| 349 | L | A | 0.3947 | |
| 350 | N | A | 0.0368 | |
| 351 | V | A | 0.0000 | |
| 352 | T | A | 0.0000 | |
| 353 | S | A | 0.0587 | |
| 354 | G | A | 0.0000 | |
| 355 | A | A | 0.0000 | |
| 356 | L | A | -0.4635 | |
| 357 | L | A | -0.2050 | |
| 358 | C | A | 0.0000 | |
| 359 | S | A | 0.0000 | |
| 360 | Q | A | -1.3554 | |
| 361 | A | A | -0.3044 | |
| 362 | L | A | -0.5793 | |
| 363 | C | A | 0.0000 | |
| 364 | S | A | -1.3092 | |
| 365 | G | A | -1.3266 | |
| 366 | H | A | -1.5777 | |
| 367 | G | A | 0.0000 | |
| 368 | R | A | -0.6545 | |
| 369 | C | A | 0.0000 | |
| 370 | V | A | -0.4428 | |
| 371 | R | A | 0.0000 | |
| 372 | R | A | -1.0425 | |
| 373 | T | A | -0.9506 | |
| 374 | S | A | -0.7493 | |
| 375 | H | A | -1.1468 | |
| 376 | P | A | -1.4854 | |
| 377 | K | A | -2.0547 | |
| 378 | A | A | -0.9977 | |
| 379 | L | A | -0.2912 | |
| 380 | L | A | 0.0000 | |
| 381 | L | A | 0.1628 | |
| 382 | L | A | 0.0000 | |
| 383 | N | A | -0.8738 | |
| 384 | P | A | -0.5388 | |
| 385 | A | A | -0.3522 | |
| 386 | S | A | -0.6901 | |
| 387 | F | A | 0.0000 | |
| 388 | S | A | -0.9130 | |
| 389 | I | A | -0.2977 | |
| 390 | Q | A | -0.7625 | |
| 391 | L | A | -0.0472 | |
| 392 | T | A | -0.2982 | |
| 393 | P | A | -0.6451 | |
| 394 | G | A | -0.7904 | |
| 395 | G | A | -0.8228 | |
| 396 | G | A | -0.6391 | |
| 397 | P | A | -0.4206 | |
| 398 | L | A | -0.2476 | |
| 399 | S | A | -0.2330 | |
| 400 | L | A | -0.4703 | |
| 401 | R | A | -1.8952 | |
| 402 | G | A | -1.1461 | |
| 403 | A | A | -0.2279 | |
| 404 | L | A | 0.1165 | |
| 405 | S | A | -0.1792 | |
| 406 | L | A | 0.3423 | |
| 407 | E | A | -1.6564 | |
| 408 | D | A | -1.2048 | |
| 409 | Q | A | -0.7064 | |
| 410 | A | A | -0.8166 | |
| 411 | Q | A | -1.2981 | |
| 412 | M | A | 0.0000 | |
| 413 | A | A | -0.3916 | |
| 414 | V | A | 0.2929 | |
| 415 | E | A | -0.6690 | |
| 416 | F | A | 0.0000 | |
| 417 | K | A | -0.9845 | |
| 418 | C | A | -0.6692 | |
| 419 | R | A | -0.7912 | |
| 420 | C | A | -0.8562 | |
| 421 | Y | A | 0.0000 | |
| 422 | P | A | -0.2231 | |
| 423 | G | A | -0.2463 | |
| 424 | W | A | -1.4521 | |
| 425 | Q | A | -1.6695 | |
| 426 | A | A | -1.1017 | |
| 427 | P | A | -0.7499 | |
| 428 | W | A | -0.7693 | |
| 429 | C | A | 0.0000 | |
| 430 | E | A | -3.0825 | |
| 431 | R | A | -3.4828 | |
| 432 | K | A | -2.6912 | |
| 433 | S | A | -0.8480 | |
| 434 | M | A | 0.9256 | |
| 435 | W | A | 1.4279 | |
| 436 | T | A | 0.4472 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine