Project name: SH3_G131Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131Y
Energy difference between WT (input) and mutated protein (by FoldX)	5.22907 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.013
• Maximal score value: 1.7964
• Average score: -0.912
• Total score value: -51.9858

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5137
86	L	A	0.7912
87	F	A	0.9195
88	V	A	0.4291
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5623
93	Y	A	-1.9359
94	E	A	-2.6508
95	A	A	-2.5716
96	R	A	-2.9873
97	T	A	-2.6624
98	E	A	-3.0130
99	D	A	-2.8829
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1262
103	F	A	0.0000
104	H	A	-2.7309
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6401
109	F	A	0.0000
110	Q	A	-0.5102
111	I	A	-0.0017
112	L	A	0.2086
113	N	A	-0.8941
114	S	A	-1.1821
115	S	A	-1.5971
116	E	A	-2.5613
117	G	A	-2.1340
118	D	A	-2.4458
119	W	A	-1.0433
120	W	A	-0.9713
121	E	A	-0.9815
122	A	A	0.0000
123	R	A	-1.5066
124	S	A	0.0000
125	L	A	0.0924
126	T	A	-0.4435
127	T	A	-0.7641
128	G	A	-1.2126
129	E	A	-1.9622
130	T	A	-1.3321
131	Y	A	-0.9220	mutated: GA131Y
132	Y	A	-0.6021
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9228
136	N	A	-1.1505
137	Y	A	-0.1244
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7739
141	V	A	1.7964