Project name: SH3_I111T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111T
Energy difference between WT (input) and mutated protein (by FoldX)	3.29823 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7932
• Average score: -0.9932
• Total score value: -56.6134

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4463
86	L	A	0.6999
87	F	A	0.8364
88	V	A	0.4267
89	A	A	0.0000
90	L	A	-0.1522
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4592
107	E	A	-1.3093
108	K	A	-0.6891
109	F	A	0.0000
110	Q	A	-0.7067
111	T	A	-0.4628	mutated: IA111T
112	L	A	-0.0499
113	N	A	-1.0138
114	S	A	-1.2994
115	S	A	-1.6118
116	E	A	-2.5704
117	G	A	-2.1429
118	D	A	-2.4520
119	W	A	-1.1101
120	W	A	-1.1581
121	E	A	-1.2643
122	A	A	0.0000
123	R	A	-1.8109
124	S	A	0.0000
125	L	A	0.0582
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.7380
131	G	A	-1.5384
132	Y	A	-0.9140
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9696
136	N	A	-1.1500
137	Y	A	-0.1251
138	V	A	0.0000
139	A	A	0.4128
140	P	A	0.7716
141	V	A	1.7932