Project name: SGLT 2 human mini itasser

Chain sequence(s)	A: MEEHTEAGSAPEMGAQKALIDNPADILVIAAYFLLVIGVGLWSMCRTNRGTVGGYFLAGRSMVWWPVGASLFASNIGSGHFVGLAGTGAASGLAVAGFEWNALFVVLLLGWLFAPVYLTAGVITMPQYLRKRFGGRRIRLYLSVLSLFLYIFTKISVDMFSGAVFIQQALGWNIYASVIALLGITMIYTVTGGLAALMYTDTVQTFVILGGACILMGYAFHEVGGYSGLFDKYLGAATSLTVSEDPAVGNISSFCYRPRPDSYHLLRHPVTGDLPWPALLLGLTIVSGWYWCSDQVIVQRCLAGKSLTHIKAGCILCGYLKLTPMFLMVMPGMISRILYPDEVACVVPEVCRRVCGTEVGCSNIAYPRLVVKLMPNGLRGLMLAVMLAALMSSLASIFNSSSTLFTMDIYTRLRPRAGDRELLLVGRLWVVFIVVVSVAWLPVVQAAQGGQLFDYIQAVSSYLAPPVSAVFVLALFVPRVNEQGAFWGLIGGLLMGLARLIPEFSFGSGSCVQPSACPAFLCGVHYLYFAIVLFFCSGLLTLTVSLCTAPIPRKHLHRLVFSLRHSKEEREDLDADEQQGSSLPVQNGCPESAMEMNEPQAPAPSLFRQCLLWFCGMSRGGVGSPPPLTQEEAAAAARRLEDISEDPSWARVVNLNALLMMAVAVFLWGFYA
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.5162
• Maximal score value: 3.1596
• Average score: 0.0162
• Total score value: 10.9132

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	-1.0853
2	E	A	-2.9119
3	E	A	-3.4640
4	H	A	-2.6445
5	T	A	-2.1839
6	E	A	-2.5550
7	A	A	-1.2999
8	G	A	-1.7987
9	S	A	-2.0626
10	A	A	-1.8118
11	P	A	-1.8031
12	E	A	-1.8940
13	M	A	-0.7203
14	G	A	-1.3099
15	A	A	-0.9391
16	Q	A	-1.2292
17	K	A	-1.0026
18	A	A	-0.1471
19	L	A	1.0272
20	I	A	1.0688
21	D	A	0.2260
22	N	A	0.1178
23	P	A	0.5470
24	A	A	0.4387
25	D	A	0.0000
26	I	A	1.5717
27	L	A	2.3396
28	V	A	1.7615
29	I	A	0.0000
30	A	A	2.0255
31	A	A	2.0876
32	Y	A	0.0000
33	F	A	2.5301
34	L	A	3.0387
35	L	A	3.1115
36	V	A	2.4712
37	I	A	3.0595
38	G	A	2.5582
39	V	A	2.3705
40	G	A	0.0000
41	L	A	2.5540
42	W	A	2.0083
43	S	A	1.0211
44	M	A	0.0000
45	C	A	-0.0121
46	R	A	-1.6745
47	T	A	-1.2741
48	N	A	-0.9853
49	R	A	-2.0066
50	G	A	-1.1801
51	T	A	-0.3455
52	V	A	1.4113
53	G	A	0.3286
54	G	A	-0.3718
55	Y	A	0.6898
56	F	A	1.2566
57	L	A	0.5987
58	A	A	-0.4625
59	G	A	-0.9764
60	R	A	-2.0910
61	S	A	-1.0457
62	M	A	-0.4166
63	V	A	0.0000
64	W	A	0.5740
65	W	A	0.9821
66	P	A	0.0000
67	V	A	0.0000
68	G	A	0.0000
69	A	A	0.0000
70	S	A	-0.0670
71	L	A	0.0000
72	F	A	0.0000
73	A	A	-0.2252
74	S	A	-0.2720
75	N	A	0.0000
76	I	A	0.0000
77	G	A	-0.3122
78	S	A	0.0000
79	G	A	-0.2925
80	H	A	-0.3365
81	F	A	0.0000
82	V	A	0.0000
83	G	A	0.0000
84	L	A	0.0000
85	A	A	0.0000
86	G	A	0.0000
87	T	A	0.0000
88	G	A	0.0000
89	A	A	0.0000
90	A	A	0.2266
91	S	A	0.0000
92	G	A	0.0000
93	L	A	0.0000
94	A	A	0.0000
95	V	A	0.0029
96	A	A	0.0000
97	G	A	0.0000
98	F	A	0.0000
99	E	A	0.0000
100	W	A	0.0000
101	N	A	0.5375
102	A	A	0.0000
103	L	A	0.0000
104	F	A	0.8787
105	V	A	0.0000
106	V	A	0.0000
107	L	A	0.0000
108	L	A	0.6200
109	L	A	0.0000
110	G	A	0.0000
111	W	A	-0.2846
112	L	A	-0.0853
113	F	A	0.0000
114	A	A	0.0000
115	P	A	0.0000
116	V	A	0.0000
117	Y	A	0.0000
118	L	A	0.2902
119	T	A	-0.5801
120	A	A	-0.3113
121	G	A	-0.1467
122	V	A	0.0000
123	I	A	1.4278
124	T	A	0.4437
125	M	A	-0.1736
126	P	A	-0.6708
127	Q	A	-0.9052
128	Y	A	0.0000
129	L	A	0.0000
130	R	A	-2.1689
131	K	A	-1.6964
132	R	A	0.0000
133	F	A	0.0000
134	G	A	-1.5203
135	G	A	-1.8182
136	R	A	-2.0847
137	R	A	-1.4435
138	I	A	0.0000
139	R	A	-0.7514
140	L	A	0.0609
141	Y	A	0.7536
142	L	A	0.0000
143	S	A	0.0000
144	V	A	1.5053
145	L	A	0.9597
146	S	A	0.6174
147	L	A	0.0000
148	F	A	0.9823
149	L	A	0.0000
150	Y	A	0.0000
151	I	A	1.1275
152	F	A	0.9011
153	T	A	0.0000
154	K	A	-0.2749
155	I	A	0.0000
156	S	A	0.0000
157	V	A	0.0000
158	D	A	-0.3471
159	M	A	0.0000
160	F	A	0.0000
161	S	A	0.0000
162	G	A	0.0000
163	A	A	0.0000
164	V	A	0.0000
165	F	A	0.0000
166	I	A	0.0000
167	Q	A	-0.0949
168	Q	A	-0.1582
169	A	A	0.1486
170	L	A	0.1784
171	G	A	-0.0404
172	W	A	0.1593
173	N	A	-0.4007
174	I	A	0.4780
175	Y	A	1.4687
176	A	A	0.7476
177	S	A	0.0000
178	V	A	0.0000
179	I	A	1.4321
180	A	A	0.7274
181	L	A	0.0000
182	L	A	0.0000
183	G	A	1.0359
184	I	A	1.4388
185	T	A	0.0000
186	M	A	1.8542
187	I	A	2.5451
188	Y	A	1.4687
189	T	A	0.0000
190	V	A	1.7216
191	T	A	0.8621
192	G	A	0.7308
193	G	A	-0.0540
194	L	A	-0.1638
195	A	A	-0.1850
196	A	A	0.5335
197	L	A	0.0000
198	M	A	0.9295
199	Y	A	1.3777
200	T	A	0.8307
201	D	A	-0.3229
202	T	A	0.6313
203	V	A	0.8560
204	Q	A	0.1075
205	T	A	0.0000
206	F	A	0.7465
207	V	A	0.0000
208	I	A	0.0000
209	L	A	0.0000
210	G	A	0.6021
211	G	A	0.0000
212	A	A	0.0000
213	C	A	0.7637
214	I	A	1.2046
215	L	A	0.0000
216	M	A	0.6556
217	G	A	0.7352
218	Y	A	0.5124
219	A	A	0.0000
220	F	A	0.0000
221	H	A	-0.1032
222	E	A	-0.4536
223	V	A	0.1693
224	G	A	0.0864
225	G	A	0.0988
226	Y	A	0.8756
227	S	A	0.3763
228	G	A	0.6648
229	L	A	1.5488
230	F	A	1.7444
231	D	A	-0.1810
232	K	A	0.0000
233	Y	A	0.8174
234	L	A	0.5714
235	G	A	0.0833
236	A	A	0.0000
237	A	A	0.0000
238	T	A	0.0312
239	S	A	-0.1743
240	L	A	-0.0266
241	T	A	-0.2248
242	V	A	0.0664
243	S	A	0.0000
244	E	A	0.0000
245	D	A	-1.3423
246	P	A	-0.4524
247	A	A	-0.2364
248	V	A	0.0000
249	G	A	0.0652
250	N	A	-0.1986
251	I	A	1.3875
252	S	A	0.7497
253	S	A	0.2057
254	F	A	0.5456
255	C	A	0.0000
256	Y	A	0.0050
257	R	A	-0.8828
258	P	A	-1.6201
259	R	A	-2.6795
260	P	A	-1.9620
261	D	A	-2.2726
262	S	A	-1.3815
263	Y	A	-0.1137
264	H	A	0.0000
265	L	A	0.7172
266	L	A	1.0052
267	R	A	-0.1513
268	H	A	0.0000
269	P	A	0.0000
270	V	A	-0.1223
271	T	A	0.0000
272	G	A	0.0000
273	D	A	-0.0839
274	L	A	0.0000
275	P	A	0.0000
276	W	A	1.0984
277	P	A	1.1217
278	A	A	0.0000
279	L	A	1.4225
280	L	A	2.1567
281	L	A	1.6856
282	G	A	0.0000
283	L	A	0.0000
284	T	A	0.6705
285	I	A	0.0000
286	V	A	0.0000
287	S	A	0.0000
288	G	A	0.0260
289	W	A	0.0581
290	Y	A	-0.0219
291	W	A	0.0000
292	C	A	0.0000
293	S	A	0.0000
294	D	A	-0.5975
295	Q	A	0.0000
296	V	A	0.5853
297	I	A	0.8168
298	V	A	0.0000
299	Q	A	0.0000
300	R	A	0.6978
301	C	A	0.0000
302	L	A	0.0000
303	A	A	-0.0490
304	G	A	0.0000
305	K	A	-1.3694
306	S	A	-0.5189
307	L	A	0.0419
308	T	A	-0.5030
309	H	A	-0.3471
310	I	A	0.0000
311	K	A	0.0000
312	A	A	-0.1765
313	G	A	0.0000
314	C	A	0.0000
315	I	A	0.0000
316	L	A	0.0000
317	C	A	0.0000
318	G	A	0.0000
319	Y	A	0.2065
320	L	A	0.0000
321	K	A	0.0000
322	L	A	0.0000
323	T	A	0.0000
324	P	A	0.0000
325	M	A	0.0000
326	F	A	0.3036
327	L	A	0.0000
328	M	A	0.0000
329	V	A	0.0000
330	M	A	0.0000
331	P	A	0.0000
332	G	A	0.0000
333	M	A	0.0000
334	I	A	0.0000
335	S	A	0.0000
336	R	A	-0.2335
337	I	A	0.0000
338	L	A	0.0000
339	Y	A	-0.2747
340	P	A	-0.6187
341	D	A	-0.3001
342	E	A	-0.3944
343	V	A	1.7678
344	A	A	1.1675
345	C	A	1.3584
346	V	A	2.6473
347	V	A	2.5001
348	P	A	1.2335
349	E	A	0.9430
350	V	A	1.6468
351	C	A	0.7825
352	R	A	0.4067
353	R	A	-0.3325
354	V	A	1.1670
355	C	A	0.4589
356	G	A	0.2174
357	T	A	-0.0139
358	E	A	-0.4475
359	V	A	0.5837
360	G	A	-0.2956
361	C	A	-0.3909
362	S	A	0.0000
363	N	A	0.0000
364	I	A	0.0000
365	A	A	-0.0060
366	Y	A	0.0000
367	P	A	0.0000
368	R	A	0.0000
369	L	A	0.0000
370	V	A	0.0000
371	V	A	0.0000
372	K	A	-0.0024
373	L	A	-0.0194
374	M	A	0.0000
375	P	A	-0.5837
376	N	A	-1.2262
377	G	A	-0.1155
378	L	A	0.3794
379	R	A	-0.1238
380	G	A	0.0000
381	L	A	0.9101
382	M	A	0.0000
383	L	A	0.0000
384	A	A	0.0000
385	V	A	0.0000
386	M	A	0.0000
387	L	A	0.0000
388	A	A	0.0000
389	A	A	0.0000
390	L	A	0.0000
391	M	A	0.4340
392	S	A	-0.1064
393	S	A	-0.2485
394	L	A	0.0000
395	A	A	0.0000
396	S	A	-0.2294
397	I	A	-0.1924
398	F	A	0.0000
399	N	A	-0.4151
400	S	A	-0.2488
401	S	A	0.0000
402	S	A	0.0000
403	T	A	0.4432
404	L	A	0.6462
405	F	A	0.7682
406	T	A	0.0000
407	M	A	0.8845
408	D	A	0.0000
409	I	A	0.8452
410	Y	A	0.5615
411	T	A	-0.4820
412	R	A	-0.2540
413	L	A	0.6465
414	R	A	-1.1240
415	P	A	-1.3807
416	R	A	-2.4803
417	A	A	-2.1832
418	G	A	-2.3750
419	D	A	-2.7859
420	R	A	-2.3906
421	E	A	-1.2950
422	L	A	0.0000
423	L	A	0.0544
424	L	A	1.3798
425	V	A	1.1787
426	G	A	0.8416
427	R	A	1.3078
428	L	A	2.4117
429	W	A	1.7430
430	V	A	0.0000
431	V	A	2.0957
432	F	A	2.3605
433	I	A	0.0000
434	V	A	0.0000
435	V	A	2.8235
436	V	A	2.1240
437	S	A	0.0000
438	V	A	1.8234
439	A	A	1.4831
440	W	A	1.2999
441	L	A	0.0000
442	P	A	0.9284
443	V	A	0.6538
444	V	A	0.0000
445	Q	A	-0.4564
446	A	A	-0.2737
447	A	A	-0.5062
448	Q	A	-1.2642
449	G	A	-0.7941
450	G	A	-0.5014
451	Q	A	-0.1732
452	L	A	0.0000
453	F	A	0.0000
454	D	A	-0.1019
455	Y	A	0.1759
456	I	A	0.4492
457	Q	A	0.2237
458	A	A	0.3946
459	V	A	0.5392
460	S	A	0.0000
461	S	A	0.0000
462	Y	A	0.8272
463	L	A	0.0000
464	A	A	0.0000
465	P	A	0.0000
466	P	A	0.0000
467	V	A	0.4387
468	S	A	0.0000
469	A	A	0.0000
470	V	A	0.0000
471	F	A	0.0000
472	V	A	0.8751
473	L	A	0.0000
474	A	A	0.0000
475	L	A	0.0000
476	F	A	1.3253
477	V	A	0.4121
478	P	A	0.0000
479	R	A	-1.0141
480	V	A	0.0000
481	N	A	-0.6287
482	E	A	-0.7343
483	Q	A	-0.5409
484	G	A	0.0000
485	A	A	0.0000
486	F	A	0.6310
487	W	A	1.1945
488	G	A	0.0000
489	L	A	0.0000
490	I	A	1.5790
491	G	A	1.2579
492	G	A	0.0000
493	L	A	1.4735
494	L	A	2.0977
495	M	A	1.3780
496	G	A	0.0000
497	L	A	2.0633
498	A	A	1.3852
499	R	A	0.9235
500	L	A	1.4258
501	I	A	1.9102
502	P	A	0.3037
503	E	A	-1.0394
504	F	A	0.1142
505	S	A	-0.0805
506	F	A	0.0000
507	G	A	0.1303
508	S	A	-0.0784
509	G	A	0.2307
510	S	A	0.0143
511	C	A	0.2684
512	V	A	0.4640
513	Q	A	-0.1734
514	P	A	-0.2621
515	S	A	-0.3108
516	A	A	0.0228
517	C	A	0.2734
518	P	A	0.0722
519	A	A	0.0931
520	F	A	0.3893
521	L	A	0.0000
522	C	A	0.1037
523	G	A	0.0000
524	V	A	0.0000
525	H	A	0.0000
526	Y	A	0.5588
527	L	A	0.0000
528	Y	A	0.5273
529	F	A	0.0000
530	A	A	0.0000
531	I	A	0.0000
532	V	A	0.8551
533	L	A	0.0000
534	F	A	1.2127
535	F	A	1.8584
536	C	A	1.5187
537	S	A	0.0000
538	G	A	0.0000
539	L	A	2.1633
540	L	A	1.6474
541	T	A	0.0000
542	L	A	1.0220
543	T	A	0.9740
544	V	A	0.0000
545	S	A	0.0000
546	L	A	0.6347
547	C	A	0.9571
548	T	A	0.3014
549	A	A	-0.1296
550	P	A	0.1559
551	I	A	0.0233
552	P	A	-1.1905
553	R	A	-2.4384
554	K	A	-1.8824
555	H	A	-1.1607
556	L	A	0.0000
557	H	A	-1.2988
558	R	A	-1.4328
559	L	A	0.0000
560	V	A	0.0000
561	F	A	0.0000
562	S	A	0.0000
563	L	A	0.0000
564	R	A	0.0000
565	H	A	0.0000
566	S	A	0.0000
567	K	A	-0.8795
568	E	A	0.0000
569	E	A	-2.5710
570	R	A	-3.5009
571	E	A	-2.7728
572	D	A	0.0000
573	L	A	0.0000
574	D	A	-1.4232
575	A	A	-0.9272
576	D	A	-0.7326
577	E	A	0.0000
578	Q	A	0.0000
579	Q	A	0.0000
580	G	A	-0.5801
581	S	A	-0.5080
582	S	A	0.0000
583	L	A	-0.7895
584	P	A	-1.1934
585	V	A	-0.8280
586	Q	A	-1.3548
587	N	A	-2.1374
588	G	A	-1.6436
589	C	A	0.0000
590	P	A	0.0000
591	E	A	-2.5175
592	S	A	-1.6808
593	A	A	-1.1616
594	M	A	-0.6422
595	E	A	-1.8267
596	M	A	0.0000
597	N	A	-1.4316
598	E	A	-2.4291
599	P	A	-1.5281
600	Q	A	-1.4540
601	A	A	-1.1152
602	P	A	-0.6521
603	A	A	0.0000
604	P	A	-1.0091
605	S	A	-0.9721
606	L	A	0.0000
607	F	A	0.0000
608	R	A	-1.3111
609	Q	A	-0.6313
610	C	A	0.0000
611	L	A	1.0487
612	L	A	1.3641
613	W	A	0.0000
614	F	A	0.0000
615	C	A	0.8748
616	G	A	0.1050
617	M	A	0.0000
618	S	A	-0.8623
619	R	A	-1.3261
620	G	A	-0.6392
621	G	A	0.0000
622	V	A	-0.0713
623	G	A	0.2261
624	S	A	-0.1476
625	P	A	0.0000
626	P	A	-0.3469
627	P	A	-0.4865
628	L	A	0.5630
629	T	A	-0.1500
630	Q	A	0.0000
631	E	A	-2.0918
632	E	A	-1.7119
633	A	A	-1.1952
634	A	A	-1.6996
635	A	A	-1.8537
636	A	A	0.0000
637	A	A	-1.5319
638	R	A	-2.8186
639	R	A	-2.1144
640	L	A	0.0000
641	E	A	-1.9534
642	D	A	-3.0592
643	I	A	-2.3001
644	S	A	-1.3376
645	E	A	-3.5162
646	D	A	-3.3290
647	P	A	-2.2654
648	S	A	0.0000
649	W	A	0.0000
650	A	A	0.0000
651	R	A	-2.3038
652	V	A	-0.4393
653	V	A	0.0000
654	N	A	-0.2372
655	L	A	0.7200
656	N	A	0.0000
657	A	A	0.0000
658	L	A	-0.4995
659	L	A	-0.4247
660	M	A	0.0000
661	M	A	0.0000
662	A	A	0.0000
663	V	A	2.2270
664	A	A	0.0000
665	V	A	2.5989
666	F	A	2.3279
667	L	A	0.0000
668	W	A	1.6592
669	G	A	1.6110
670	F	A	3.1596
671	Y	A	2.6096
672	A	A	1.8313