Project name: SH3_Y137V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA137V
Energy difference between WT (input) and mutated protein (by FoldX)	2.96215 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9683
• Total score value: -55.1921

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9027
88	V	A	0.4032
89	A	A	0.0000
90	L	A	-0.2029
91	Y	A	-0.6081
92	D	A	-2.5638
93	Y	A	-1.9144
94	E	A	-2.6217
95	A	A	-2.6138
96	R	A	-2.9797
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1903
103	F	A	0.0000
104	H	A	-2.7161
105	K	A	-2.4219
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8890
114	S	A	-1.1810
115	S	A	-1.5972
116	E	A	-2.5613
117	G	A	-2.1362
118	D	A	-2.4481
119	W	A	-1.1296
120	W	A	-1.0594
121	E	A	-1.1547
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.5001
132	Y	A	-0.8696
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9817
136	N	A	-1.2397
137	V	A	-0.2989	mutated: YA137V
138	V	A	0.0000
139	A	A	0.3771
140	P	A	0.7757
141	V	A	1.7964