Project name: SH3_E107R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107R
Energy difference between WT (input) and mutated protein (by FoldX)	1.22031 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.105
• Maximal score value: 1.8095
• Average score: -0.9871
• Total score value: -56.262

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.8627
88	V	A	0.3142
89	A	A	0.0000
90	L	A	-0.1931
91	Y	A	-0.6134
92	D	A	-2.6117
93	Y	A	-1.9374
94	E	A	-2.6527
95	A	A	-2.6332
96	R	A	-2.9881
97	T	A	-2.6630
98	E	A	-3.1050
99	D	A	-3.0454
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2242
103	F	A	0.0000
104	H	A	-2.8491
105	K	A	-2.4985
106	G	A	-1.5691
107	R	A	-1.7434	mutated: EA107R
108	K	A	-0.8490
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1536
122	A	A	0.0000
123	R	A	-1.7132
124	S	A	0.0000
125	L	A	-0.0371
126	T	A	-0.5659
127	T	A	-0.8705
128	G	A	-1.3527
129	E	A	-2.2396
130	T	A	-1.6946
131	G	A	-1.5021
132	Y	A	-0.8705
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4289
140	P	A	0.7757
141	V	A	1.8095