Project name: SH3_P140S

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	PA140S
Energy difference between WT (input) and mutated protein (by FoldX)	1.91809 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8396
• Average score: -0.953
• Total score value: -54.3214

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5413
86	L	A	0.8271
87	F	A	0.9828
88	V	A	0.4642
89	A	A	0.0000
90	L	A	-0.1463
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4541
107	E	A	-1.3036
108	K	A	-0.6249
109	F	A	0.0000
110	Q	A	-0.4946
111	I	A	-0.0494
112	L	A	0.1439
113	N	A	-0.8873
114	S	A	-1.1789
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0552
121	E	A	-1.1525
122	A	A	0.0000
123	R	A	-1.7150
124	S	A	0.0000
125	L	A	0.0621
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6925
131	G	A	-1.4990
132	Y	A	-0.8671
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9158
136	N	A	-1.1492
137	Y	A	-0.1197
138	V	A	0.0000
139	A	A	0.4642
140	S	A	0.8563	mutated: PA140S
141	V	A	1.8396