Project name: SH3_Q110V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	QA110V
Energy difference between WT (input) and mutated protein (by FoldX)	-0.393171 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7941
• Average score: -0.88
• Total score value: -50.1605

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.7931
86	L	A	1.2439
87	F	A	1.2521
88	V	A	0.6087
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3114
108	K	A	-0.4074
109	F	A	0.0000
110	V	A	0.7879	mutated: QA110V
111	I	A	0.5726
112	L	A	0.5480
113	N	A	-0.6688
114	S	A	-1.1565
115	S	A	-1.5782
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-0.9313
121	E	A	-0.9969
122	A	A	0.0000
123	R	A	-1.5778
124	S	A	0.0000
125	L	A	0.2530
126	T	A	-0.5287
127	T	A	-0.8791
128	G	A	-1.3110
129	E	A	-2.3490
130	T	A	-1.6629
131	G	A	-1.5629
132	Y	A	-0.9022
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9261
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4147
140	P	A	0.9161
141	V	A	1.7941