Project name: SH3_F87A

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87A
Energy difference between WT (input) and mutated protein (by FoldX)	4.21369 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7185
• Average score: -0.9774
• Total score value: -55.7111

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4770
86	L	A	0.7008
87	A	A	0.0000	mutated: FA87A
88	V	A	0.3192
89	A	A	0.0000
90	L	A	-0.1781
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4856
107	E	A	-1.3428
108	K	A	-0.7056
109	F	A	0.0000
110	Q	A	-0.5164
111	I	A	-0.0015
112	L	A	0.1998
113	N	A	-0.8365
114	S	A	-1.1276
115	S	A	-1.5802
116	E	A	-2.5415
117	G	A	-2.1147
118	D	A	-2.4189
119	W	A	-1.0569
120	W	A	-0.9460
121	E	A	-1.0865
122	A	A	0.0000
123	R	A	-1.6863
124	S	A	0.0000
125	L	A	0.0356
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6835
131	G	A	-1.4758
132	Y	A	-0.8277
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9142
136	N	A	-1.1402
137	Y	A	-0.1506
138	V	A	0.0000
139	A	A	0.3386
140	P	A	0.6770
141	V	A	1.7185