Project name: SH3_L125S

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125S
Energy difference between WT (input) and mutated protein (by FoldX)	1.43771 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8526
• Average score: -1.051
• Total score value: -59.9072

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5830
86	L	A	0.7718
87	F	A	0.8231
88	V	A	0.2549
89	A	A	0.0000
90	L	A	-0.1706
91	Y	A	-0.5854
92	D	A	-2.5609
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7340
105	K	A	-2.4173
106	G	A	-1.4746
107	E	A	-1.5954
108	K	A	-1.0701
109	F	A	0.0000
110	Q	A	-0.9198
111	I	A	-0.1098
112	L	A	0.0871
113	N	A	-0.9104
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0794
121	E	A	-1.1870
122	A	A	0.0000
123	R	A	-2.1252
124	S	A	0.0000
125	S	A	-1.2911	mutated: LA125S
126	T	A	-1.1339
127	T	A	-1.2088
128	G	A	-1.7942
129	E	A	-2.4648
130	T	A	-1.8786
131	G	A	-1.5075
132	Y	A	-0.8724
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1511
137	Y	A	-0.1361
138	V	A	0.0000
139	A	A	0.4302
140	P	A	0.8071
141	V	A	1.8526