Project name: SH3_L86Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86Q
Energy difference between WT (input) and mutated protein (by FoldX)	1.22723 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.4379
• Average score: -1.1039
• Total score value: -62.9202

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.1379
86	Q	A	-0.6229	mutated: LA86Q
87	F	A	0.1960
88	V	A	0.0490
89	A	A	0.0000
90	L	A	-0.1176
91	Y	A	-0.5354
92	D	A	-2.5201
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6774
105	K	A	-2.3032
106	G	A	-1.2393
107	E	A	-1.2985
108	K	A	-1.2301
109	F	A	0.0000
110	Q	A	-1.5664
111	I	A	-0.5111
112	L	A	0.2435
113	N	A	-0.7863
114	S	A	-1.1057
115	S	A	-1.5585
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0925
121	E	A	-1.2154
122	A	A	0.0000
123	R	A	-2.5086
124	S	A	0.0000
125	L	A	-0.6041
126	T	A	-0.6631
127	T	A	-0.9847
128	G	A	-1.6457
129	E	A	-2.4427
130	T	A	-1.9605
131	G	A	-1.5775
132	Y	A	-0.9012
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9195
136	N	A	-1.1511
137	Y	A	-0.1379
138	V	A	0.0000
139	A	A	0.2381
140	P	A	0.3726
141	V	A	1.4379