Project name: SH3_V88P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88P
Energy difference between WT (input) and mutated protein (by FoldX)	5.90742 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.6694
• Average score: -0.9959
• Total score value: -56.7654

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4736
86	L	A	0.6882
87	F	A	0.7076
88	P	A	-0.0013	mutated: VA88P
89	A	A	0.0000
90	L	A	-0.2263
91	Y	A	-0.6110
92	D	A	-2.5526
93	Y	A	-1.9271
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7747
105	K	A	-2.4784
106	G	A	-1.5883
107	E	A	-1.4495
108	K	A	-0.8502
109	F	A	0.0000
110	Q	A	-0.5701
111	I	A	-0.0596
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7192
124	S	A	0.0000
125	L	A	0.0041
126	T	A	-0.4785
127	T	A	-0.8232
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9205
136	N	A	-1.1453
137	Y	A	-0.1794
138	V	A	0.0000
139	A	A	0.2824
140	P	A	0.6606
141	V	A	1.6694