Project name: SH3_V141A

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141A
Energy difference between WT (input) and mutated protein (by FoldX)	1.29482 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 0.4334
• Average score: -1.0252
• Total score value: -58.4359

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.2016
86	L	A	0.4334
87	F	A	0.4294
88	V	A	-0.0003
89	A	A	0.0000
90	L	A	-0.1165
91	Y	A	-0.5507
92	D	A	-2.5306
93	Y	A	-1.9318
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6729
105	K	A	-2.3123
106	G	A	-1.4345
107	E	A	-1.1658
108	K	A	-0.7301
109	F	A	0.0000
110	Q	A	-0.4417
111	I	A	-0.0193
112	L	A	0.1369
113	N	A	-0.8907
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0581
121	E	A	-1.1563
122	A	A	0.0000
123	R	A	-1.6653
124	S	A	0.0000
125	L	A	0.1945
126	T	A	-0.3803
127	T	A	-0.7817
128	G	A	-1.3323
129	E	A	-2.2256
130	T	A	-1.6856
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.1405
138	V	A	0.0000
139	A	A	-0.0010
140	P	A	-0.0289
141	A	A	0.1449	mutated: VA141A