Project name: SH3_W119V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119V
Energy difference between WT (input) and mutated protein (by FoldX)	1.5727 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1033
• Maximal score value: 1.7964
• Average score: -0.9554
• Total score value: -54.4581

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9233
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1470
91	Y	A	-0.5669
92	D	A	-2.5455
93	Y	A	-1.9068
94	E	A	-2.6312
95	A	A	-2.6225
96	R	A	-2.9819
97	T	A	-2.6608
98	E	A	-3.1033
99	D	A	-3.0444
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1974
103	F	A	0.0000
104	H	A	-2.7210
105	K	A	-2.4006
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5088
111	I	A	-0.0573
112	L	A	0.1425
113	N	A	-0.8647
114	S	A	-1.1471
115	S	A	-1.5864
116	E	A	-2.5458
117	G	A	-2.1329
118	D	A	-2.3717
119	V	A	-0.9045	mutated: WA119V
120	W	A	-0.9604
121	E	A	-1.1100
122	A	A	0.0000
123	R	A	-1.7154
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6934
131	G	A	-1.4726
132	Y	A	-0.8082
133	I	A	0.0000
134	P	A	-0.4977
135	S	A	-0.8743
136	N	A	-1.1276
137	Y	A	-0.1049
138	V	A	0.0000
139	A	A	0.4153
140	P	A	0.7757
141	V	A	1.7964