Project name: SH3_S135W

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135W
Energy difference between WT (input) and mutated protein (by FoldX)	1.24542 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7861
• Average score: -0.9183
• Total score value: -52.3452

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5104
86	L	A	0.7789
87	F	A	0.9794
88	V	A	0.4352
89	A	A	0.0000
90	L	A	-0.1162
91	Y	A	-0.5443
92	D	A	-2.5415
93	Y	A	-1.9107
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.3896
106	G	A	-1.4617
107	E	A	-1.3124
108	K	A	-0.6519
109	F	A	0.0000
110	Q	A	-0.5201
111	I	A	-0.0077
112	L	A	0.1363
113	N	A	-0.8919
114	S	A	-1.0835
115	S	A	-1.5850
116	E	A	-2.5425
117	G	A	-2.0157
118	D	A	-2.2481
119	W	A	-0.9099
120	W	A	-0.9241
121	E	A	-1.0975
122	A	A	0.0000
123	R	A	-1.7199
124	S	A	0.0000
125	L	A	0.0560
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6925
131	G	A	-1.5067
132	Y	A	-0.8028
133	I	A	0.0000
134	P	A	0.0000
135	W	A	-0.3141	mutated: SA135W
136	N	A	-0.8190
137	Y	A	0.1798
138	V	A	0.0000
139	A	A	0.5279
140	P	A	0.7625
141	V	A	1.7861