Project name: SH3_T97D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA97D
Energy difference between WT (input) and mutated protein (by FoldX)	1.30684 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -4.3793
• Maximal score value: 1.7964
• Average score: -1.0453
• Total score value: -59.5847

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9316
94	E	A	-2.8496
95	A	A	-3.1256
96	R	A	-3.8156
97	D	A	-4.3793	mutated: TA97D
98	E	A	-3.9436
99	D	A	-3.6042
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.4062
103	F	A	0.0000
104	H	A	-2.7283
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0569
112	L	A	0.1429
113	N	A	-0.8884
114	S	A	-1.1800
115	S	A	-1.5964
116	E	A	-2.5600
117	G	A	-2.1338
118	D	A	-2.4461
119	W	A	-1.1073
120	W	A	-1.0579
121	E	A	-1.1540
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6927
131	G	A	-1.4998
132	Y	A	-0.8689
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9221
136	N	A	-1.1512
137	Y	A	-0.1249
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964