| Chain sequence(s) |
A: DNREIVMKYIHYKLSQRGYEWDVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSM C: KKLSECLRRIGDELDSNMELQRMIAD B: DNREIVMKYIHYKLSQRGYEWDVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSM D: KKLSECLRRIGDELDSNMELQRMIAD |
| Distance of aggregation | 5 Å |
| Dynamic mode | No |
| Mutated residues | EB29D |
| Energy difference between WT (input) and mutated protein (by FoldX) | -0.599821 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 10 | D | A | -2.0750 | |
| 11 | N | A | 0.0000 | |
| 12 | R | A | -2.1827 | |
| 13 | E | A | -1.4425 | |
| 14 | I | A | 0.0000 | |
| 15 | V | A | 0.0000 | |
| 16 | M | A | 0.1623 | |
| 17 | K | A | -0.6516 | |
| 18 | Y | A | 0.0000 | |
| 19 | I | A | 0.0000 | |
| 20 | H | A | -0.3427 | |
| 21 | Y | A | 0.1419 | |
| 22 | K | A | 0.0000 | |
| 23 | L | A | 0.0000 | |
| 24 | S | A | -0.3270 | |
| 25 | Q | A | -0.7205 | |
| 26 | R | A | -1.9934 | |
| 27 | G | A | -0.7704 | |
| 28 | Y | A | -0.0935 | |
| 29 | E | A | -1.7259 | |
| 30 | W | A | -0.4962 | |
| 31 | D | A | -1.7577 | |
| 92 | V | A | 1.9426 | |
| 93 | V | A | 0.5413 | |
| 94 | H | A | 0.3179 | |
| 95 | L | A | 1.4045 | |
| 96 | T | A | 0.0000 | |
| 97 | L | A | 0.0000 | |
| 98 | R | A | -0.9777 | |
| 99 | Q | A | -0.7446 | |
| 100 | A | A | 0.0000 | |
| 101 | G | A | 0.0000 | |
| 102 | D | A | -0.3800 | |
| 103 | D | A | -0.3364 | |
| 104 | F | A | 0.0000 | |
| 105 | S | A | 0.0000 | |
| 106 | R | A | -1.8982 | |
| 107 | R | A | -0.6347 | |
| 108 | Y | A | 0.0000 | |
| 109 | R | A | -1.9246 | |
| 110 | R | A | -2.4702 | |
| 111 | D | A | -2.0791 | |
| 112 | F | A | -0.0533 | |
| 113 | A | A | -0.2287 | |
| 114 | E | A | -1.8091 | |
| 115 | M | A | 0.0000 | |
| 116 | S | A | -0.1118 | |
| 117 | S | A | -0.3105 | |
| 118 | Q | A | -0.4985 | |
| 119 | L | A | 0.0000 | |
| 120 | H | A | -0.9349 | |
| 121 | L | A | 0.0000 | |
| 122 | T | A | -0.0592 | |
| 123 | P | A | 0.1931 | |
| 124 | F | A | 1.9116 | |
| 125 | T | A | 0.0000 | |
| 126 | A | A | 0.0000 | |
| 127 | R | A | -0.5337 | |
| 128 | G | A | 0.0000 | |
| 129 | R | A | -0.1796 | |
| 130 | F | A | 0.0000 | |
| 131 | A | A | 0.0137 | |
| 132 | T | A | 0.0000 | |
| 133 | V | A | 0.1848 | |
| 134 | V | A | 0.0000 | |
| 135 | E | A | -0.5899 | |
| 136 | E | A | -0.4628 | |
| 137 | L | A | 0.0000 | |
| 138 | F | A | -0.0363 | |
| 139 | R | A | -1.4834 | |
| 140 | D | A | -2.0444 | |
| 141 | G | A | -0.4512 | |
| 142 | V | A | 0.2877 | |
| 143 | N | A | -0.1363 | |
| 144 | W | A | 0.0000 | |
| 145 | G | A | 0.0000 | |
| 146 | R | A | -0.1882 | |
| 147 | I | A | 0.0000 | |
| 148 | V | A | 0.0000 | |
| 149 | A | A | 0.0000 | |
| 150 | F | A | 0.0000 | |
| 151 | F | A | 0.0000 | |
| 152 | E | A | 0.0000 | |
| 153 | F | A | 0.0000 | |
| 154 | G | A | 0.0000 | |
| 155 | G | A | 0.0000 | |
| 156 | V | A | 0.1877 | |
| 157 | M | A | 0.0000 | |
| 158 | C | A | 0.0000 | |
| 159 | V | A | 0.0000 | |
| 160 | E | A | -0.3132 | |
| 161 | S | A | 0.0000 | |
| 162 | V | A | 0.1212 | |
| 163 | N | A | -1.3482 | |
| 164 | R | A | -1.2383 | |
| 165 | E | A | -1.9197 | |
| 166 | M | A | -0.2485 | |
| 167 | S | A | -0.1201 | |
| 168 | P | A | -0.2528 | |
| 169 | L | A | 0.0304 | |
| 170 | V | A | 0.0000 | |
| 171 | D | A | -1.6903 | |
| 172 | N | A | -1.0484 | |
| 173 | I | A | 0.0000 | |
| 174 | A | A | 0.0000 | |
| 175 | L | A | 0.4914 | |
| 176 | W | A | 0.2181 | |
| 177 | M | A | 0.0000 | |
| 178 | T | A | 0.0000 | |
| 179 | E | A | -1.4207 | |
| 180 | Y | A | -0.1325 | |
| 181 | L | A | 0.0000 | |
| 182 | N | A | -1.4275 | |
| 183 | R | A | -2.2122 | |
| 184 | H | A | -1.3320 | |
| 185 | L | A | 0.0000 | |
| 186 | H | A | -1.0672 | |
| 187 | T | A | -0.2128 | |
| 188 | W | A | 0.1775 | |
| 189 | I | A | 0.0000 | |
| 190 | Q | A | -1.5393 | |
| 191 | D | A | -2.1607 | |
| 192 | N | A | -1.2223 | |
| 193 | G | A | -0.6268 | |
| 194 | G | A | -0.2810 | |
| 195 | W | A | -0.1935 | |
| 196 | D | A | -1.7296 | |
| 197 | A | A | -0.3703 | |
| 198 | F | A | 0.0000 | |
| 199 | V | A | -0.2598 | |
| 200 | E | A | -1.7491 | |
| 201 | L | A | -0.1441 | |
| 202 | Y | A | 0.0000 | |
| 203 | G | A | -0.3589 | |
| 204 | P | A | -0.1007 | |
| 205 | S | A | 0.1312 | |
| 206 | M | A | 1.0432 | |
| 10 | D | B | -1.7894 | |
| 11 | N | B | 0.0000 | |
| 12 | R | B | -1.1543 | |
| 13 | E | B | -1.9479 | |
| 14 | I | B | 0.0000 | |
| 15 | V | B | 0.0000 | |
| 16 | M | B | -0.2706 | |
| 17 | K | B | -1.3225 | |
| 18 | Y | B | 0.0000 | |
| 19 | I | B | 0.0000 | |
| 20 | H | B | -0.4891 | |
| 21 | Y | B | 0.0738 | |
| 22 | K | B | 0.0000 | |
| 23 | L | B | 0.0000 | |
| 24 | S | B | -0.3781 | |
| 25 | Q | B | -1.0899 | |
| 26 | R | B | -2.0618 | |
| 27 | G | B | -0.7568 | |
| 28 | Y | B | -0.0071 | |
| 29 | D | B | -1.6847 | mutated: EB29D |
| 30 | W | B | -0.5011 | |
| 31 | D | B | -1.7596 | |
| 92 | V | B | 1.8289 | |
| 93 | V | B | 0.7302 | |
| 94 | H | B | 0.2628 | |
| 95 | L | B | 1.3533 | |
| 96 | T | B | 0.0000 | |
| 97 | L | B | 0.0000 | |
| 98 | R | B | -0.9878 | |
| 99 | Q | B | -0.5970 | |
| 100 | A | B | 0.0000 | |
| 101 | G | B | 0.0000 | |
| 102 | D | B | -0.2740 | |
| 103 | D | B | -0.3171 | |
| 104 | F | B | 0.0000 | |
| 105 | S | B | -0.3613 | |
| 106 | R | B | -1.9043 | |
| 107 | R | B | -0.6453 | |
| 108 | Y | B | 0.0000 | |
| 109 | R | B | -1.9826 | |
| 110 | R | B | -2.4803 | |
| 111 | D | B | -2.0917 | |
| 112 | F | B | -0.2979 | |
| 113 | A | B | -0.2432 | |
| 114 | E | B | -1.8090 | |
| 115 | M | B | 0.0000 | |
| 116 | S | B | -0.1046 | |
| 117 | S | B | -0.2726 | |
| 118 | Q | B | -0.2959 | |
| 119 | L | B | 0.0000 | |
| 120 | H | B | -0.9557 | |
| 121 | L | B | -0.0071 | |
| 122 | T | B | -0.0182 | |
| 123 | P | B | 0.2326 | |
| 124 | F | B | 1.9173 | |
| 125 | T | B | 0.3611 | |
| 126 | A | B | 0.0000 | |
| 127 | R | B | -0.5392 | |
| 128 | G | B | 0.0000 | |
| 129 | R | B | 0.0000 | |
| 130 | F | B | 0.0000 | |
| 131 | A | B | 0.0065 | |
| 132 | T | B | -0.0072 | |
| 133 | V | B | 0.0000 | |
| 134 | V | B | 0.0000 | |
| 135 | E | B | -0.3629 | |
| 136 | E | B | -0.8067 | |
| 137 | L | B | 0.0000 | |
| 138 | F | B | -0.1490 | |
| 139 | R | B | -2.2007 | |
| 140 | D | B | -2.2016 | |
| 141 | G | B | -0.6436 | |
| 142 | V | B | 0.2226 | |
| 143 | N | B | -0.1375 | |
| 144 | W | B | 0.0000 | |
| 145 | G | B | 0.0000 | |
| 146 | R | B | 0.0000 | |
| 147 | I | B | 0.0000 | |
| 148 | V | B | 0.0000 | |
| 149 | A | B | 0.0000 | |
| 150 | F | B | 0.0000 | |
| 151 | F | B | 0.0000 | |
| 152 | E | B | 0.0000 | |
| 153 | F | B | 0.0000 | |
| 154 | G | B | 0.0000 | |
| 155 | G | B | 0.0000 | |
| 156 | V | B | 0.2194 | |
| 157 | M | B | 0.0000 | |
| 158 | C | B | 0.0000 | |
| 159 | V | B | 0.1916 | |
| 160 | E | B | 0.0000 | |
| 161 | S | B | 0.0000 | |
| 162 | V | B | 0.1426 | |
| 163 | N | B | -1.5470 | |
| 164 | R | B | -2.4199 | |
| 165 | E | B | -2.1399 | |
| 166 | M | B | -0.2448 | |
| 167 | S | B | -0.0983 | |
| 168 | P | B | -0.2508 | |
| 169 | L | B | 0.1049 | |
| 170 | V | B | 0.0000 | |
| 171 | D | B | -1.5578 | |
| 172 | N | B | -0.9476 | |
| 173 | I | B | 0.0000 | |
| 174 | A | B | 0.0000 | |
| 175 | L | B | 0.6464 | |
| 176 | W | B | 0.2459 | |
| 177 | M | B | 0.0000 | |
| 178 | T | B | 0.0000 | |
| 179 | E | B | -1.1287 | |
| 180 | Y | B | -0.0339 | |
| 181 | L | B | 0.0000 | |
| 182 | N | B | -0.8991 | |
| 183 | R | B | -2.1186 | |
| 184 | H | B | -1.3336 | |
| 185 | L | B | 0.0000 | |
| 186 | H | B | -0.7282 | |
| 187 | T | B | -0.1480 | |
| 188 | W | B | 0.0220 | |
| 189 | I | B | 0.0000 | |
| 190 | Q | B | -1.5399 | |
| 191 | D | B | -2.1464 | |
| 192 | N | B | -1.3103 | |
| 193 | G | B | -0.6404 | |
| 194 | G | B | -0.3016 | |
| 195 | W | B | -0.1935 | |
| 196 | D | B | -1.7604 | |
| 197 | A | B | -0.3157 | |
| 198 | F | B | 0.0000 | |
| 199 | V | B | 0.0343 | |
| 200 | E | B | -1.7197 | |
| 201 | L | B | -0.0589 | |
| 202 | Y | B | 0.0000 | |
| 203 | G | B | -0.4029 | |
| 204 | P | B | -0.0996 | |
| 205 | S | B | 0.1316 | |
| 206 | M | B | 1.0439 | |
| 57 | K | C | -1.8884 | |
| 58 | K | C | -1.3101 | |
| 59 | L | C | -0.0413 | |
| 60 | S | C | 0.0000 | |
| 61 | E | C | -0.2607 | |
| 62 | C | C | 0.0000 | |
| 63 | L | C | 0.0000 | |
| 64 | R | C | -1.3986 | |
| 65 | R | C | -1.8642 | |
| 66 | I | C | 0.0000 | |
| 67 | G | C | 0.0000 | |
| 68 | D | C | -0.5252 | |
| 69 | E | C | -0.4666 | |
| 70 | L | C | 0.0000 | |
| 71 | D | C | -0.4691 | |
| 72 | S | C | -0.3862 | |
| 73 | N | C | -0.5870 | |
| 74 | M | C | 0.0000 | |
| 75 | E | C | -1.6978 | |
| 76 | L | C | 0.6602 | |
| 77 | Q | C | -0.4681 | |
| 78 | R | C | -0.6692 | |
| 79 | M | C | 1.4177 | |
| 80 | I | C | 2.2220 | |
| 81 | A | C | 0.1035 | |
| 82 | D | C | -1.7787 | |
| 57 | K | D | -1.7706 | |
| 58 | K | D | -0.6982 | |
| 59 | L | D | 0.0000 | |
| 60 | S | D | -0.1941 | |
| 61 | E | D | -0.7550 | |
| 62 | C | D | 0.0000 | |
| 63 | L | D | 0.0000 | |
| 64 | R | D | -0.7839 | |
| 65 | R | D | -1.9889 | |
| 66 | I | D | 0.0000 | |
| 67 | G | D | 0.0000 | |
| 68 | D | D | -1.0566 | |
| 69 | E | D | -0.5812 | |
| 70 | L | D | 0.0000 | |
| 71 | D | D | -0.4855 | |
| 72 | S | D | -0.4041 | |
| 73 | N | D | -1.0106 | |
| 74 | M | D | 0.0000 | |
| 75 | E | D | -1.6142 | |
| 76 | L | D | 0.5922 | |
| 77 | Q | D | -0.3042 | |
| 78 | R | D | -0.4729 | |
| 79 | M | D | 0.8976 | |
| 80 | I | D | 2.1735 | |
| 81 | A | D | 0.0941 | |
| 82 | D | D | -1.6873 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine