| Chain sequence(s) |
A: EKPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPS C: KPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPS B: KPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPS E: GPLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLGELSKVLSISTERHRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTVTANAVANAAI D: PRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPS |
| Distance of aggregation | 5 Å |
| Dynamic mode | No |
| Mutated residues | IA161T |
| Energy difference between WT (input) and mutated protein (by FoldX) | 2.8137 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 7 | G | E | -0.5125 | |
| 8 | P | E | -0.0548 | |
| 9 | L | E | 1.1701 | |
| 10 | D | E | -1.3503 | |
| 11 | L | E | 0.4703 | |
| 12 | S | E | -0.3039 | |
| 13 | R | E | -2.1948 | |
| 14 | D | E | -2.2831 | |
| 15 | E | E | -1.1296 | |
| 16 | C | E | -0.2200 | |
| 17 | K | E | -1.7330 | |
| 18 | R | E | -1.9539 | |
| 19 | I | E | 0.6851 | |
| 20 | L | E | 1.2975 | |
| 21 | R | E | -0.2393 | |
| 22 | K | E | -0.6272 | |
| 23 | L | E | 1.2606 | |
| 24 | E | E | -0.0179 | |
| 25 | L | E | -0.2262 | |
| 26 | E | E | -1.7846 | |
| 27 | A | E | -0.3061 | |
| 28 | Y | E | 0.0000 | |
| 29 | A | E | -0.0432 | |
| 30 | G | E | -0.1935 | |
| 31 | V | E | 0.4413 | |
| 32 | I | E | 0.0000 | |
| 33 | S | E | -0.0335 | |
| 34 | A | E | 0.0640 | |
| 35 | L | E | 0.1604 | |
| 36 | R | E | 0.0000 | |
| 37 | A | E | -0.1050 | |
| 38 | Q | E | -0.7143 | |
| 39 | G | E | 0.0000 | |
| 40 | D | E | -0.3475 | |
| 41 | L | E | 0.0000 | |
| 42 | T | E | -0.3387 | |
| 43 | K | E | -2.1576 | |
| 44 | E | E | -2.1903 | |
| 45 | K | E | -0.7436 | |
| 46 | K | E | -1.7023 | |
| 47 | D | E | -1.8242 | |
| 48 | L | E | 0.7383 | |
| 49 | L | E | 0.0000 | |
| 50 | G | E | -0.8742 | |
| 51 | E | E | -1.8399 | |
| 52 | L | E | -0.0942 | |
| 53 | S | E | -0.2982 | |
| 54 | K | E | -1.3790 | |
| 55 | V | E | 1.4994 | |
| 56 | L | E | 0.5023 | |
| 57 | S | E | -0.1733 | |
| 58 | I | E | 0.0000 | |
| 59 | S | E | -0.1212 | |
| 60 | T | E | -0.0909 | |
| 61 | E | E | 0.0000 | |
| 62 | R | E | -0.4264 | |
| 63 | H | E | -0.1802 | |
| 64 | R | E | 0.0000 | |
| 65 | A | E | 0.0080 | |
| 66 | E | E | 0.0000 | |
| 67 | V | E | 0.0000 | |
| 68 | R | E | -0.3143 | |
| 69 | R | E | -0.7663 | |
| 70 | A | E | 0.0000 | |
| 71 | V | E | 0.0000 | |
| 72 | N | E | -1.3107 | |
| 73 | D | E | -0.7731 | |
| 74 | E | E | -2.2002 | |
| 75 | R | E | -2.1473 | |
| 76 | L | E | -0.1645 | |
| 77 | T | E | -0.1872 | |
| 78 | T | E | 0.1055 | |
| 79 | I | E | 0.9625 | |
| 80 | A | E | 0.0000 | |
| 81 | H | E | -0.9084 | |
| 82 | N | E | -1.2005 | |
| 83 | M | E | 0.7801 | |
| 84 | S | E | -0.0366 | |
| 85 | G | E | -0.2853 | |
| 86 | P | E | -0.5070 | |
| 87 | N | E | -1.3199 | |
| 88 | S | E | 0.0000 | |
| 89 | S | E | -0.0993 | |
| 90 | S | E | -0.4284 | |
| 91 | E | E | -1.1896 | |
| 92 | W | E | 0.0000 | |
| 93 | S | E | 0.2098 | |
| 94 | I | E | 1.1120 | |
| 95 | E | E | 0.0352 | |
| 96 | G | E | 0.0000 | |
| 97 | R | E | -0.9459 | |
| 98 | R | E | -0.3465 | |
| 99 | L | E | 0.0000 | |
| 100 | V | E | 0.0000 | |
| 101 | P | E | 0.0000 | |
| 102 | L | E | 0.0000 | |
| 103 | M | E | 0.0000 | |
| 104 | P | E | -0.2438 | |
| 105 | R | E | -0.9464 | |
| 106 | L | E | 0.0000 | |
| 107 | V | E | 0.2593 | |
| 108 | P | E | -0.3473 | |
| 109 | Q | E | -1.2331 | |
| 110 | T | E | -0.2286 | |
| 111 | A | E | 0.0000 | |
| 112 | F | E | 0.0000 | |
| 113 | T | E | 0.0000 | |
| 114 | V | E | 0.0000 | |
| 115 | T | E | 0.0000 | |
| 116 | A | E | 0.0000 | |
| 117 | N | E | -1.2712 | |
| 118 | A | E | -0.1414 | |
| 119 | V | E | 0.4605 | |
| 120 | A | E | -0.0905 | |
| 121 | N | E | -1.2524 | |
| 122 | A | E | -0.1649 | |
| 123 | A | E | 0.4391 | |
| 124 | I | E | 2.0233 | |
| 108 | E | A | -2.1344 | |
| 109 | K | A | -2.0672 | |
| 110 | P | A | -0.8152 | |
| 111 | R | A | -1.8749 | |
| 112 | G | A | 0.0000 | |
| 113 | F | A | 0.2873 | |
| 114 | A | A | 0.0469 | |
| 115 | R | A | -0.4148 | |
| 116 | G | A | -0.4622 | |
| 117 | L | A | -0.0601 | |
| 118 | E | A | -1.7602 | |
| 119 | P | A | -0.4317 | |
| 120 | E | A | -0.4982 | |
| 121 | R | A | -0.4430 | |
| 122 | I | A | 0.0000 | |
| 123 | I | A | 0.0000 | |
| 124 | G | A | 0.0000 | |
| 125 | A | A | 0.0000 | |
| 126 | T | A | -0.3355 | |
| 127 | D | A | -1.8154 | |
| 128 | S | A | -0.5028 | |
| 129 | S | A | -0.3237 | |
| 130 | G | A | -0.7450 | |
| 131 | E | A | -1.3510 | |
| 132 | L | A | -0.0331 | |
| 133 | M | A | 0.2100 | |
| 134 | F | A | 0.0000 | |
| 135 | L | A | 0.0000 | |
| 136 | M | A | 0.0000 | |
| 137 | K | A | 0.0000 | |
| 138 | W | A | 0.0000 | |
| 139 | K | A | -1.1675 | |
| 140 | N | A | -1.4424 | |
| 141 | S | A | -0.3499 | |
| 142 | D | A | -0.8263 | |
| 143 | E | A | -1.9095 | |
| 144 | A | A | 0.0000 | |
| 145 | D | A | 0.0000 | |
| 146 | L | A | 0.1809 | |
| 147 | V | A | 0.0000 | |
| 148 | P | A | -0.0146 | |
| 149 | A | A | 0.0000 | |
| 150 | K | A | -1.8727 | |
| 151 | E | A | -1.2484 | |
| 152 | A | A | 0.0000 | |
| 153 | N | A | 0.0987 | |
| 154 | V | A | 1.4193 | |
| 155 | K | A | -1.3744 | |
| 156 | C | A | 0.0000 | |
| 157 | P | A | -0.2908 | |
| 158 | Q | A | -1.1690 | |
| 159 | V | A | -0.0137 | |
| 160 | V | A | 0.0000 | |
| 161 | T | A | 0.0000 | mutated: IA161T |
| 162 | S | A | -0.0985 | |
| 163 | F | A | 0.0000 | |
| 164 | Y | A | 0.0000 | |
| 165 | E | A | -0.5560 | |
| 166 | E | A | -0.2987 | |
| 167 | R | A | 0.0000 | |
| 168 | L | A | 0.0000 | |
| 169 | T | A | 0.0000 | |
| 170 | W | A | 0.0000 | |
| 171 | H | A | -0.5830 | |
| 172 | S | A | -0.3812 | |
| 173 | Y | A | 0.4341 | |
| 174 | P | A | 0.0178 | |
| 175 | S | A | -0.2184 | |
| 109 | K | B | -1.7370 | |
| 110 | P | B | -0.7381 | |
| 111 | R | B | -1.2659 | |
| 112 | G | B | 0.0000 | |
| 113 | F | B | 0.2977 | |
| 114 | A | B | 0.0340 | |
| 115 | R | B | -0.4996 | |
| 116 | G | B | -0.4614 | |
| 117 | L | B | 0.0279 | |
| 118 | E | B | -1.7444 | |
| 119 | P | B | -0.5568 | |
| 120 | E | B | -1.3328 | |
| 121 | R | B | -2.0260 | |
| 122 | I | B | 0.0000 | |
| 123 | I | B | 0.7879 | |
| 124 | G | B | 0.0000 | |
| 125 | A | B | 0.0000 | |
| 126 | T | B | 0.0000 | |
| 127 | D | B | -0.5213 | |
| 128 | S | B | -0.2911 | |
| 129 | S | B | -0.3287 | |
| 130 | G | B | -0.7212 | |
| 131 | E | B | -1.2491 | |
| 132 | L | B | 0.0000 | |
| 133 | M | B | 0.1496 | |
| 134 | F | B | 0.0000 | |
| 135 | L | B | 0.0000 | |
| 136 | M | B | 0.0000 | |
| 137 | K | B | -0.2716 | |
| 138 | W | B | 0.0000 | |
| 139 | K | B | -1.1150 | |
| 140 | N | B | -1.4391 | |
| 141 | S | B | -0.5887 | |
| 142 | D | B | -2.1304 | |
| 143 | E | B | -2.1507 | |
| 144 | A | B | 0.0000 | |
| 145 | D | B | 0.0000 | |
| 146 | L | B | 0.0000 | |
| 147 | V | B | 0.0000 | |
| 148 | P | B | -0.0256 | |
| 149 | A | B | 0.0000 | |
| 150 | K | B | -1.8916 | |
| 151 | E | B | -1.3471 | |
| 152 | A | B | 0.0000 | |
| 153 | N | B | 0.0907 | |
| 154 | V | B | 1.4199 | |
| 155 | K | B | -1.3759 | |
| 156 | C | B | 0.0000 | |
| 157 | P | B | -0.2900 | |
| 158 | Q | B | -1.1681 | |
| 159 | V | B | -0.0127 | |
| 160 | V | B | 0.0000 | |
| 161 | I | B | 0.0000 | |
| 162 | S | B | -0.1701 | |
| 163 | F | B | 0.0000 | |
| 164 | Y | B | 0.0000 | |
| 165 | E | B | -1.0922 | |
| 166 | E | B | -2.0175 | |
| 167 | R | B | -0.5828 | |
| 168 | L | B | 0.3222 | |
| 169 | T | B | 0.1237 | |
| 170 | W | B | 0.3705 | |
| 171 | H | B | 0.0000 | |
| 172 | S | B | -0.1712 | |
| 173 | Y | B | 0.1403 | |
| 174 | P | B | -0.2531 | |
| 175 | S | B | -0.2600 | |
| 109 | K | C | -1.7328 | |
| 110 | P | C | -0.8310 | |
| 111 | R | C | -1.8771 | |
| 112 | G | C | 0.0000 | |
| 113 | F | C | 0.2816 | |
| 114 | A | C | 0.0513 | |
| 115 | R | C | -0.3859 | |
| 116 | G | C | -0.4360 | |
| 117 | L | C | 0.0566 | |
| 118 | E | C | -1.7403 | |
| 119 | P | C | -0.4120 | |
| 120 | E | C | -0.2845 | |
| 121 | R | C | -0.4989 | |
| 122 | I | C | 0.0000 | |
| 123 | I | C | 0.0000 | |
| 124 | G | C | -0.0476 | |
| 125 | A | C | 0.0000 | |
| 126 | T | C | 0.0000 | |
| 127 | D | C | -1.3371 | |
| 128 | S | C | -0.4634 | |
| 129 | S | C | -0.3381 | |
| 130 | G | C | -0.7636 | |
| 131 | E | C | -1.5978 | |
| 132 | L | C | 0.0000 | |
| 133 | M | C | 0.1216 | |
| 134 | F | C | 0.0000 | |
| 135 | L | C | 0.0000 | |
| 136 | M | C | 0.0000 | |
| 137 | K | C | 0.0000 | |
| 138 | W | C | 0.0000 | |
| 139 | K | C | -0.6517 | |
| 140 | N | C | -1.3800 | |
| 141 | S | C | -0.4706 | |
| 142 | D | C | -1.4952 | |
| 143 | E | C | -2.0331 | |
| 144 | A | C | 0.0000 | |
| 145 | D | C | 0.0000 | |
| 146 | L | C | 0.2050 | |
| 147 | V | C | 0.0000 | |
| 148 | P | C | -0.0206 | |
| 149 | A | C | 0.0000 | |
| 150 | K | C | -1.9346 | |
| 151 | E | C | -1.5843 | |
| 152 | A | C | 0.0000 | |
| 153 | N | C | 0.0863 | |
| 154 | V | C | 1.4170 | |
| 155 | K | C | -1.3744 | |
| 156 | C | C | 0.0000 | |
| 157 | P | C | -0.2373 | |
| 158 | Q | C | -0.7943 | |
| 159 | V | C | 0.1132 | |
| 160 | V | C | 0.0000 | |
| 161 | I | C | 0.0000 | |
| 162 | S | C | -0.0582 | |
| 163 | F | C | 0.0000 | |
| 164 | Y | C | 0.0000 | |
| 165 | E | C | -0.5239 | |
| 166 | E | C | -1.0981 | |
| 167 | R | C | 0.0000 | |
| 168 | L | C | 0.0000 | |
| 169 | T | C | 0.0000 | |
| 170 | W | C | 0.1999 | |
| 171 | H | C | -0.0776 | |
| 172 | S | C | -0.0307 | |
| 173 | Y | C | 0.1064 | |
| 174 | P | C | -0.2634 | |
| 175 | S | C | -0.2594 | |
| 110 | P | D | -0.5846 | |
| 111 | R | D | -1.8343 | |
| 112 | G | D | 0.0000 | |
| 113 | F | D | 0.3241 | |
| 114 | A | D | 0.0307 | |
| 115 | R | D | -0.5420 | |
| 116 | G | D | -0.4888 | |
| 117 | L | D | -0.0737 | |
| 118 | E | D | -1.7619 | |
| 119 | P | D | -0.5620 | |
| 120 | E | D | -1.3832 | |
| 121 | R | D | -2.0352 | |
| 122 | I | D | 0.0000 | |
| 123 | I | D | 0.3702 | |
| 124 | G | D | 0.0000 | |
| 125 | A | D | 0.0000 | |
| 126 | T | D | 0.0000 | |
| 127 | D | D | -1.3535 | |
| 128 | S | D | -0.4977 | |
| 129 | S | D | -0.3382 | |
| 130 | G | D | -0.7976 | |
| 131 | E | D | -1.6264 | |
| 132 | L | D | -0.2575 | |
| 133 | M | D | 0.1502 | |
| 134 | F | D | 0.0000 | |
| 135 | L | D | 0.0000 | |
| 136 | M | D | 0.0000 | |
| 137 | K | D | -0.3976 | |
| 138 | W | D | 0.0000 | |
| 139 | K | D | -0.9961 | |
| 140 | N | D | -1.4199 | |
| 141 | S | D | -0.6010 | |
| 142 | D | D | -1.9110 | |
| 143 | E | D | -0.9493 | |
| 144 | A | D | -0.1815 | |
| 145 | D | D | -0.1226 | |
| 146 | L | D | 0.3262 | |
| 147 | V | D | 0.0000 | |
| 148 | P | D | -0.0264 | |
| 149 | A | D | 0.0000 | |
| 150 | K | D | -1.9034 | |
| 151 | E | D | -1.4211 | |
| 152 | A | D | 0.0000 | |
| 153 | N | D | 0.1830 | |
| 154 | V | D | 1.4367 | |
| 155 | K | D | -1.3765 | |
| 156 | C | D | 0.0000 | |
| 157 | P | D | -0.2761 | |
| 158 | Q | D | -1.1666 | |
| 159 | V | D | -0.0040 | |
| 160 | V | D | 0.0000 | |
| 161 | I | D | 0.0000 | |
| 162 | S | D | -0.1275 | |
| 163 | F | D | 0.1629 | |
| 164 | Y | D | 0.0000 | |
| 165 | E | D | -1.0209 | |
| 166 | E | D | -2.1231 | |
| 167 | R | D | -1.2567 | |
| 168 | L | D | 0.0226 | |
| 169 | T | D | 0.0940 | |
| 170 | W | D | 0.3379 | |
| 171 | H | D | -0.1349 | |
| 172 | S | D | -0.2063 | |
| 173 | Y | D | 0.1406 | |
| 174 | P | D | -0.1719 | |
| 175 | S | D | -0.2442 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine