Project name: SH3_T130V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA130V
Energy difference between WT (input) and mutated protein (by FoldX)	-0.836689 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1025
• Maximal score value: 1.7964
• Average score: -0.878
• Total score value: -50.0456

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4749
86	L	A	0.7235
87	F	A	0.8751
88	V	A	0.4066
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6257
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1025
99	D	A	-2.8949
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.0873
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3042
108	K	A	-0.6772
109	F	A	0.0000
110	Q	A	-0.5294
111	I	A	0.0393
112	L	A	0.3664
113	N	A	-0.8475
114	S	A	-1.1488
115	S	A	-1.5802
116	E	A	-2.5577
117	G	A	-2.1323
118	D	A	-2.4426
119	W	A	-1.0988
120	W	A	-1.0416
121	E	A	-0.8746
122	A	A	0.0000
123	R	A	-1.1943
124	S	A	0.0000
125	L	A	0.1980
126	T	A	-0.4280
127	T	A	-0.6006
128	G	A	-0.9662
129	E	A	-1.6041
130	V	A	-0.5354	mutated: TA130V
131	G	A	-0.9153
132	Y	A	-0.6131
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9186
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7547
141	V	A	1.7964