Project name: SH3_K108N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	KA108N
Energy difference between WT (input) and mutated protein (by FoldX)	1.73759 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8948
• Average score: -0.9119
• Total score value: -51.9809

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5603
86	L	A	0.9957
87	F	A	1.1100
88	V	A	0.6465
89	A	A	0.0000
90	L	A	-0.1031
91	Y	A	-0.5704
92	D	A	-2.5554
93	Y	A	-1.9318
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6696
105	K	A	-2.3337
106	G	A	-1.3203
107	E	A	-1.0715
108	N	A	-0.1659	mutated: KA108N
109	F	A	0.0000
110	Q	A	-0.3683
111	I	A	0.0080
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.6198
124	S	A	0.0000
125	L	A	0.3013
126	T	A	-0.3293
127	T	A	-0.7499
128	G	A	-1.3277
129	E	A	-2.2246
130	T	A	-1.6820
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.1260
138	V	A	0.0000
139	A	A	0.4786
140	P	A	0.8507
141	V	A	1.8948