Project name: SH3_I111Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111Y
Energy difference between WT (input) and mutated protein (by FoldX)	4.17069 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7882
• Average score: -0.9253
• Total score value: -52.7432

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5902
86	L	A	0.8757
87	F	A	0.9861
88	V	A	0.4133
89	A	A	0.0000
90	L	A	-0.1540
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4610
107	E	A	-1.3174
108	K	A	-0.6135
109	F	A	0.0000
110	Q	A	-0.3108
111	Y	A	0.4205	mutated: IA111Y
112	L	A	0.3768
113	N	A	-0.7253
114	S	A	-1.0163
115	S	A	-1.5688
116	E	A	-2.5421
117	G	A	-2.1192
118	D	A	-2.4320
119	W	A	-1.0917
120	W	A	-0.9309
121	E	A	-1.0192
122	A	A	0.0000
123	R	A	-1.6259
124	S	A	0.0000
125	L	A	0.0207
126	T	A	-0.4881
127	T	A	-0.8294
128	G	A	-1.3692
129	E	A	-2.2470
130	T	A	-1.6478
131	G	A	-1.4544
132	Y	A	-0.8110
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8627
136	N	A	-1.1508
137	Y	A	-0.1271
138	V	A	0.0000
139	A	A	0.4076
140	P	A	0.7628
141	V	A	1.7882