Project name: SH3_Y137Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA137Q
Energy difference between WT (input) and mutated protein (by FoldX)	1.75946 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9898
• Total score value: -56.4205

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.8618
88	V	A	0.3487
89	A	A	0.0000
90	L	A	-0.3162
91	Y	A	-0.6999
92	D	A	-2.6231
93	Y	A	-1.9866
94	E	A	-2.6275
95	A	A	-2.6168
96	R	A	-2.9810
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1942
103	F	A	0.0000
104	H	A	-2.7191
105	K	A	-2.4712
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0567
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1798
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1346
118	D	A	-2.4453
119	W	A	-1.1621
120	W	A	-1.0567
121	E	A	-1.1535
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4995
132	Y	A	-0.8680
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0934
136	N	A	-1.4098
137	Q	A	-0.6538	mutated: YA137Q
138	V	A	0.0000
139	A	A	0.3021
140	P	A	0.7757
141	V	A	1.7964