| Chain sequence(s) |
A: MEKYVAAMVLSAAGDALGYYNGKWEFLQDGEKIHRQLAQLGGLDALDVGRWRVSDDTVMHLATAEALVEAGKAPKLTQLYYLLAKHYQDCMEDMDGRAPGGASVHNAMQLKPGKPNGWRIPFNSHEGGCGAAMRAMCIGLRFPHHSQLDTLIQVSIESGRMTHHHPTGYLGALASALFTAYAVNSRPPLQWGKGLMELLPEAKKYIVQSGYFVEENLQHWSYFQTKWENYLKLRGILDGESAPTFPESFGVKERDQFYTSLSYSGWGGSSGHDAPMIAYDAVLAAGDSWKELAHRAFFHGGDSDSTAAIAGCWWGVMYGFKGVSPSNYEKLEYRNRLEETTARALYSLGSKEDTVISL |
| Distance of aggregation | 5 Å |
| Dynamic mode | No |
| Mutated residues | AA293T |
| Energy difference between WT (input) and mutated protein (by FoldX) | 2.75532 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | M | A | 0.4756 | |
| 2 | E | A | -1.7772 | |
| 3 | K | A | -1.6217 | |
| 4 | Y | A | 0.0000 | |
| 5 | V | A | 0.0000 | |
| 6 | A | A | 0.0000 | |
| 7 | A | A | 0.0000 | |
| 8 | M | A | 0.0000 | |
| 9 | V | A | 0.0000 | |
| 10 | L | A | 0.0000 | |
| 11 | S | A | 0.0000 | |
| 12 | A | A | 0.0000 | |
| 13 | A | A | 0.0000 | |
| 14 | G | A | 0.0000 | |
| 15 | D | A | 0.0000 | |
| 16 | A | A | 0.0000 | |
| 17 | L | A | 0.0000 | |
| 18 | G | A | 0.0000 | |
| 19 | Y | A | 0.0000 | |
| 20 | Y | A | 0.0301 | |
| 21 | N | A | -0.9466 | |
| 22 | G | A | -0.5503 | |
| 23 | K | A | -1.6112 | |
| 24 | W | A | 0.0000 | |
| 25 | E | A | 0.0000 | |
| 26 | F | A | 1.7284 | |
| 27 | L | A | 0.3203 | |
| 28 | Q | A | -1.3960 | |
| 29 | D | A | -1.4917 | |
| 30 | G | A | 0.0000 | |
| 31 | E | A | -0.6392 | |
| 32 | K | A | -1.4666 | |
| 33 | I | A | 0.0000 | |
| 34 | H | A | -0.4298 | |
| 35 | R | A | -2.0396 | |
| 36 | Q | A | -0.7677 | |
| 37 | L | A | 0.0000 | |
| 38 | A | A | -0.1727 | |
| 39 | Q | A | -1.0949 | |
| 40 | L | A | 0.4024 | |
| 41 | G | A | -0.3550 | |
| 42 | G | A | -0.1743 | |
| 43 | L | A | 0.0000 | |
| 44 | D | A | -1.2658 | |
| 45 | A | A | -0.1775 | |
| 46 | L | A | 0.0000 | |
| 47 | D | A | -0.5969 | |
| 48 | V | A | 0.0000 | |
| 49 | G | A | -0.6289 | |
| 50 | R | A | -1.8598 | |
| 51 | W | A | 0.0000 | |
| 52 | R | A | -0.5564 | |
| 53 | V | A | 0.0000 | |
| 54 | S | A | 0.0000 | |
| 55 | D | A | 0.0000 | |
| 56 | D | A | 0.0000 | |
| 57 | T | A | 0.0000 | |
| 58 | V | A | 0.0000 | |
| 59 | M | A | 0.0000 | |
| 60 | H | A | 0.0000 | |
| 61 | L | A | 0.0000 | |
| 62 | A | A | 0.0000 | |
| 63 | T | A | 0.0000 | |
| 64 | A | A | 0.0000 | |
| 65 | E | A | -0.2423 | |
| 66 | A | A | 0.0000 | |
| 67 | L | A | 0.0000 | |
| 68 | V | A | 0.1472 | |
| 69 | E | A | -1.7300 | |
| 70 | A | A | -0.3434 | |
| 71 | G | A | -0.3259 | |
| 72 | K | A | -1.2880 | |
| 73 | A | A | -0.1765 | |
| 74 | P | A | 0.0000 | |
| 75 | K | A | -1.3466 | |
| 76 | L | A | 0.0000 | |
| 77 | T | A | -0.1904 | |
| 78 | Q | A | -0.6711 | |
| 79 | L | A | 0.0000 | |
| 80 | Y | A | 0.0000 | |
| 81 | Y | A | 0.5058 | |
| 82 | L | A | 0.1581 | |
| 83 | L | A | 0.0000 | |
| 84 | A | A | 0.0000 | |
| 85 | K | A | -0.9186 | |
| 86 | H | A | -0.3299 | |
| 87 | Y | A | 0.0000 | |
| 88 | Q | A | -0.5951 | |
| 89 | D | A | -1.8239 | |
| 90 | C | A | -0.2488 | |
| 91 | M | A | 0.0000 | |
| 92 | E | A | -1.9240 | |
| 93 | D | A | -0.9043 | |
| 94 | M | A | 0.0000 | |
| 95 | D | A | -1.8753 | |
| 96 | G | A | -0.7958 | |
| 97 | R | A | 0.0000 | |
| 98 | A | A | 0.0055 | |
| 99 | P | A | -0.1261 | |
| 100 | G | A | -0.3290 | |
| 101 | G | A | -0.5063 | |
| 102 | A | A | -0.0735 | |
| 103 | S | A | 0.0000 | |
| 104 | V | A | 0.2116 | |
| 105 | H | A | -0.9470 | |
| 106 | N | A | -0.4624 | |
| 107 | A | A | 0.0000 | |
| 108 | M | A | 0.3137 | |
| 109 | Q | A | -1.1175 | |
| 110 | L | A | 0.0000 | |
| 111 | K | A | -1.3425 | |
| 112 | P | A | -0.4035 | |
| 113 | G | A | -0.7995 | |
| 114 | K | A | -1.8428 | |
| 115 | P | A | -0.7871 | |
| 116 | N | A | -1.2586 | |
| 117 | G | A | -0.6663 | |
| 118 | W | A | 0.0000 | |
| 119 | R | A | -0.2697 | |
| 120 | I | A | 0.0000 | |
| 121 | P | A | -0.2135 | |
| 122 | F | A | 0.0400 | |
| 123 | N | A | -0.7688 | |
| 124 | S | A | -0.5345 | |
| 125 | H | A | -1.0291 | |
| 126 | E | A | 0.0000 | |
| 127 | G | A | -0.0708 | |
| 128 | G | A | -0.1387 | |
| 129 | C | A | 0.0000 | |
| 130 | G | A | 0.0000 | |
| 131 | A | A | 0.0000 | |
| 132 | A | A | 0.0000 | |
| 133 | M | A | 0.0000 | |
| 134 | R | A | 0.0000 | |
| 135 | A | A | 0.0000 | |
| 136 | M | A | 0.0000 | |
| 137 | C | A | 0.0000 | |
| 138 | I | A | 0.0000 | |
| 139 | G | A | 0.0000 | |
| 140 | L | A | 0.0000 | |
| 141 | R | A | 0.0000 | |
| 142 | F | A | 0.0000 | |
| 143 | P | A | 0.0000 | |
| 144 | H | A | -0.4218 | |
| 145 | H | A | -1.0727 | |
| 146 | S | A | -0.3921 | |
| 147 | Q | A | 0.0000 | |
| 148 | L | A | -0.0665 | |
| 149 | D | A | -1.7422 | |
| 150 | T | A | 0.0000 | |
| 151 | L | A | 0.0000 | |
| 152 | I | A | 0.0000 | |
| 153 | Q | A | -0.1379 | |
| 154 | V | A | 0.0000 | |
| 155 | S | A | 0.0000 | |
| 156 | I | A | 0.0000 | |
| 157 | E | A | 0.0000 | |
| 158 | S | A | 0.0000 | |
| 159 | G | A | 0.0000 | |
| 160 | R | A | 0.0000 | |
| 161 | M | A | 0.0000 | |
| 162 | T | A | 0.0000 | |
| 163 | H | A | 0.0000 | |
| 164 | H | A | 0.0000 | |
| 165 | H | A | 0.0000 | |
| 166 | P | A | 0.0000 | |
| 167 | T | A | 0.0000 | |
| 168 | G | A | 0.0000 | |
| 169 | Y | A | 0.0000 | |
| 170 | L | A | 0.0000 | |
| 171 | G | A | 0.0000 | |
| 172 | A | A | 0.0000 | |
| 173 | L | A | 0.0000 | |
| 174 | A | A | 0.0000 | |
| 175 | S | A | 0.0000 | |
| 176 | A | A | 0.0000 | |
| 177 | L | A | 0.0000 | |
| 178 | F | A | 0.0000 | |
| 179 | T | A | 0.0000 | |
| 180 | A | A | 0.0000 | |
| 181 | Y | A | 0.1467 | |
| 182 | A | A | 0.0000 | |
| 183 | V | A | 0.2814 | |
| 184 | N | A | -1.2144 | |
| 185 | S | A | -0.6390 | |
| 186 | R | A | -1.1423 | |
| 187 | P | A | -0.3397 | |
| 188 | P | A | -0.0068 | |
| 189 | L | A | 0.3106 | |
| 190 | Q | A | -0.1875 | |
| 191 | W | A | 0.0000 | |
| 192 | G | A | 0.0000 | |
| 193 | K | A | -0.6426 | |
| 194 | G | A | -0.1746 | |
| 195 | L | A | 0.0000 | |
| 196 | M | A | -0.1956 | |
| 197 | E | A | -1.7940 | |
| 198 | L | A | 0.0000 | |
| 199 | L | A | 0.0000 | |
| 200 | P | A | -0.5411 | |
| 201 | E | A | -1.2270 | |
| 202 | A | A | 0.0000 | |
| 203 | K | A | -1.0632 | |
| 204 | K | A | -1.8064 | |
| 205 | Y | A | -0.1680 | |
| 206 | I | A | 0.0000 | |
| 207 | V | A | 0.5156 | |
| 208 | Q | A | -1.0715 | |
| 209 | S | A | -0.3641 | |
| 210 | G | A | -0.3830 | |
| 211 | Y | A | 0.5733 | |
| 212 | F | A | 0.9113 | |
| 213 | V | A | 0.0737 | |
| 214 | E | A | -1.9370 | |
| 215 | E | A | -1.2434 | |
| 216 | N | A | 0.0000 | |
| 217 | L | A | 0.5341 | |
| 218 | Q | A | -1.1157 | |
| 219 | H | A | -0.5093 | |
| 220 | W | A | 0.0000 | |
| 221 | S | A | -0.1633 | |
| 222 | Y | A | 0.2340 | |
| 223 | F | A | 0.0000 | |
| 224 | Q | A | -0.4588 | |
| 225 | T | A | -0.1721 | |
| 226 | K | A | -0.2988 | |
| 227 | W | A | 0.0000 | |
| 228 | E | A | -0.7541 | |
| 229 | N | A | -1.3684 | |
| 230 | Y | A | 0.0000 | |
| 231 | L | A | 0.0000 | |
| 232 | K | A | -1.4968 | |
| 233 | L | A | 1.0079 | |
| 234 | R | A | 0.0000 | |
| 235 | G | A | -0.5220 | |
| 236 | I | A | 0.0000 | |
| 237 | L | A | 0.2270 | |
| 238 | D | A | -1.6893 | |
| 239 | G | A | 0.0000 | |
| 240 | E | A | -1.8377 | |
| 241 | S | A | -0.4238 | |
| 242 | A | A | 0.0304 | |
| 243 | P | A | -0.0589 | |
| 244 | T | A | -0.0019 | |
| 245 | F | A | 0.3974 | |
| 246 | P | A | -0.3648 | |
| 247 | E | A | -1.8784 | |
| 248 | S | A | -0.5133 | |
| 249 | F | A | 0.1317 | |
| 250 | G | A | -0.1496 | |
| 251 | V | A | -0.0922 | |
| 252 | K | A | -1.8325 | |
| 253 | E | A | -1.2984 | |
| 254 | R | A | 0.0000 | |
| 255 | D | A | -0.3929 | |
| 256 | Q | A | -1.1845 | |
| 257 | F | A | 0.0038 | |
| 258 | Y | A | 0.0000 | |
| 259 | T | A | -0.0700 | |
| 260 | S | A | -0.1939 | |
| 261 | L | A | 0.0000 | |
| 262 | S | A | 0.1825 | |
| 263 | Y | A | 1.0869 | |
| 264 | S | A | -0.0814 | |
| 265 | G | A | -0.3830 | |
| 266 | W | A | 0.3660 | |
| 267 | G | A | 0.0000 | |
| 268 | G | A | 0.0000 | |
| 269 | S | A | -0.0555 | |
| 270 | S | A | 0.0000 | |
| 271 | G | A | 0.0000 | |
| 272 | H | A | 0.0000 | |
| 273 | D | A | 0.0000 | |
| 274 | A | A | 0.0000 | |
| 275 | P | A | 0.0000 | |
| 276 | M | A | 0.0000 | |
| 277 | I | A | 0.0000 | |
| 278 | A | A | 0.0000 | |
| 279 | Y | A | 0.0000 | |
| 280 | D | A | 0.0000 | |
| 281 | A | A | 0.0000 | |
| 282 | V | A | 0.0000 | |
| 283 | L | A | 0.1785 | |
| 284 | A | A | 0.0495 | |
| 285 | A | A | 0.0000 | |
| 286 | G | A | -0.5275 | |
| 287 | D | A | -1.8372 | |
| 288 | S | A | -0.3858 | |
| 289 | W | A | 0.0000 | |
| 290 | K | A | -1.2018 | |
| 291 | E | A | -1.3151 | |
| 292 | L | A | 0.0000 | |
| 293 | T | A | 0.0000 | mutated: AA293T |
| 294 | H | A | -0.2766 | |
| 295 | R | A | -0.3352 | |
| 296 | A | A | 0.0000 | |
| 297 | F | A | 0.0000 | |
| 298 | F | A | 0.0000 | |
| 299 | H | A | 0.0000 | |
| 300 | G | A | -0.4178 | |
| 301 | G | A | 0.0000 | |
| 302 | D | A | -0.2321 | |
| 303 | S | A | 0.0000 | |
| 304 | D | A | 0.0000 | |
| 305 | S | A | 0.0000 | |
| 306 | T | A | 0.0000 | |
| 307 | A | A | 0.0000 | |
| 308 | A | A | 0.0000 | |
| 309 | I | A | 0.0000 | |
| 310 | A | A | 0.0000 | |
| 311 | G | A | 0.0000 | |
| 312 | C | A | 0.0000 | |
| 313 | W | A | 0.0000 | |
| 314 | W | A | 0.0000 | |
| 315 | G | A | 0.0000 | |
| 316 | V | A | 0.0000 | |
| 317 | M | A | 0.1741 | |
| 318 | Y | A | 0.2550 | |
| 319 | G | A | 0.0000 | |
| 320 | F | A | -0.0018 | |
| 321 | K | A | -1.7301 | |
| 322 | G | A | -0.7814 | |
| 323 | V | A | 0.0000 | |
| 324 | S | A | -0.0725 | |
| 325 | P | A | -0.2440 | |
| 326 | S | A | -0.1725 | |
| 327 | N | A | 0.0000 | |
| 328 | Y | A | -0.0729 | |
| 329 | E | A | -1.6240 | |
| 330 | K | A | -1.9556 | |
| 331 | L | A | 0.0000 | |
| 332 | E | A | -0.3599 | |
| 333 | Y | A | 0.0771 | |
| 334 | R | A | -0.8656 | |
| 335 | N | A | -1.5126 | |
| 336 | R | A | -0.8572 | |
| 337 | L | A | 0.0000 | |
| 338 | E | A | -0.7134 | |
| 339 | E | A | -1.8895 | |
| 340 | T | A | 0.0000 | |
| 341 | A | A | 0.0000 | |
| 342 | R | A | -1.8416 | |
| 343 | A | A | -0.3268 | |
| 344 | L | A | 0.0000 | |
| 345 | Y | A | 0.3722 | |
| 346 | S | A | -0.1367 | |
| 347 | L | A | 0.0000 | |
| 348 | G | A | 0.0000 | |
| 349 | S | A | -0.3936 | |
| 350 | K | A | -2.0614 | |
| 351 | E | A | -2.1973 | |
| 352 | D | A | -0.6275 | |
| 353 | T | A | -0.0871 | |
| 354 | V | A | 0.3831 | |
| 355 | I | A | 0.9155 | |
| 356 | S | A | 0.2771 | |
| 357 | L | A | 1.5085 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine