Project name: SH3_H104P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	HA104P
Energy difference between WT (input) and mutated protein (by FoldX)	3.18137 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1043
• Maximal score value: 1.7964
• Average score: -0.9056
• Total score value: -51.6208

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9252
88	V	A	0.5236
89	A	A	0.0000
90	L	A	-0.0296
91	Y	A	-0.3760
92	D	A	-2.2049
93	Y	A	-1.7372
94	E	A	-2.5344
95	A	A	-2.6309
96	R	A	-2.9871
97	T	A	-2.6623
98	E	A	-3.1043
99	D	A	-3.0447
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.0572
103	F	A	0.0000
104	P	A	-1.9405	mutated: HA104P
105	K	A	-2.0170
106	G	A	-1.2446
107	E	A	-1.0582
108	K	A	-0.5424
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7095
124	S	A	0.0000
125	L	A	0.0641
126	T	A	-0.3851
127	T	A	-0.8135
128	G	A	-1.3480
129	E	A	-2.2227
130	T	A	-1.6847
131	G	A	-1.4967
132	Y	A	-0.8690
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964