Project name: SH3_I111M

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111M
Energy difference between WT (input) and mutated protein (by FoldX)	1.50441 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7952
• Average score: -0.9787
• Total score value: -55.7874

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4768
86	L	A	0.7330
87	F	A	0.8718
88	V	A	0.4233
89	A	A	0.0000
90	L	A	-0.1516
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4585
107	E	A	-1.3143
108	K	A	-0.6819
109	F	A	0.0000
110	Q	A	-0.6319
111	M	A	-0.2568	mutated: IA111M
112	L	A	0.0786
113	N	A	-0.9367
114	S	A	-1.2379
115	S	A	-1.6027
116	E	A	-2.5674
117	G	A	-2.1403
118	D	A	-2.4499
119	W	A	-1.1077
120	W	A	-1.1044
121	E	A	-1.2020
122	A	A	0.0000
123	R	A	-1.7814
124	S	A	0.0000
125	L	A	0.0228
126	T	A	-0.4881
127	T	A	-0.8294
128	G	A	-1.3692
129	E	A	-2.2470
130	T	A	-1.7204
131	G	A	-1.5162
132	Y	A	-0.8883
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9466
136	N	A	-1.1503
137	Y	A	-0.1247
138	V	A	0.0000
139	A	A	0.4146
140	P	A	0.7716
141	V	A	1.7952