Project name: SH3_E129V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA129V
Energy difference between WT (input) and mutated protein (by FoldX)	1.03883 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0712
• Maximal score value: 1.7964
• Average score: -0.8072
• Total score value: -46.0116

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9257
88	V	A	0.4352
89	A	A	0.0000
90	L	A	-0.1493
91	Y	A	-0.5732
92	D	A	-2.5579
93	Y	A	-1.9059
94	E	A	-2.6142
95	A	A	-2.5742
96	R	A	-2.9611
97	T	A	-2.6358
98	E	A	-3.0712
99	D	A	-3.0064
100	D	A	0.0000
101	L	A	-1.3138
102	S	A	-1.7879
103	F	A	0.0000
104	H	A	-2.7241
105	K	A	-2.4020
106	G	A	-1.4521
107	E	A	-1.2954
108	K	A	-0.6366
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1340
122	A	A	0.0000
123	R	A	-0.7782
124	S	A	0.0000
125	L	A	0.5234
126	T	A	-0.0059
127	T	A	0.1123
128	G	A	0.0439
129	V	A	0.6664	mutated: EA129V
130	T	A	-0.2418
131	G	A	-0.7877
132	Y	A	-0.8208
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964