Project name: SH3_W120S

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120S
Energy difference between WT (input) and mutated protein (by FoldX)	2.66309 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8562
• Average score: -0.9476
• Total score value: -54.0107

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5685
86	L	A	0.8904
87	F	A	1.1018
88	V	A	0.5168
89	A	A	0.0000
90	L	A	-0.1305
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4373
107	E	A	-1.2814
108	K	A	-0.5821
109	F	A	0.0000
110	Q	A	-0.4565
111	I	A	-0.0275
112	L	A	0.1193
113	N	A	-0.9733
114	S	A	-1.3356
115	S	A	-1.6894
116	E	A	-2.6577
117	G	A	-2.2787
118	D	A	-2.5645
119	W	A	-1.2437
120	S	A	0.0000	mutated: WA120S
121	E	A	-1.2543
122	A	A	0.0000
123	R	A	-1.7202
124	S	A	0.0000
125	L	A	0.0772
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6862
131	G	A	-1.5222
132	Y	A	-0.9331
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0130
136	N	A	-1.1921
137	Y	A	-0.1199
138	V	A	0.0000
139	A	A	0.4675
140	P	A	0.8513
141	V	A	1.8562