Project name: SH3_Y132C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132C
Energy difference between WT (input) and mutated protein (by FoldX)	1.95057 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1271
• Maximal score value: 1.7964
• Average score: -1.0129
• Total score value: -57.7349

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9243
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6357
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1124
99	D	A	-3.1271
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2158
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.5741
111	I	A	-0.1913
112	L	A	-0.0215
113	N	A	-1.0518
114	S	A	-1.3021
115	S	A	-1.6583
116	E	A	-2.6433
117	G	A	-2.2244
118	D	A	-2.5397
119	W	A	-1.2847
120	W	A	-1.3512
121	E	A	-1.7154
122	A	A	0.0000
123	R	A	-1.8363
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3577
129	E	A	-2.2413
130	T	A	-1.8298
131	G	A	-1.7207
132	C	A	-1.1688	mutated: YA132C
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0151
136	N	A	-1.1573
137	Y	A	-0.1295
138	V	A	0.0000
139	A	A	0.4149
140	P	A	0.7757
141	V	A	1.7964