Project name: SH3_R123Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA123Y
Energy difference between WT (input) and mutated protein (by FoldX)	3.003 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1026
• Maximal score value: 1.7957
• Average score: -0.815
• Total score value: -46.4549

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4618
86	L	A	0.8375
87	F	A	0.8449
88	V	A	0.3806
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6254
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1026
99	D	A	-3.0295
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2056
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3329
108	K	A	-0.5086
109	F	A	0.0000
110	Q	A	-0.1045
111	I	A	0.6311
112	L	A	1.2346
113	N	A	-0.4634
114	S	A	-0.9567
115	S	A	-1.4821
116	E	A	-2.5377
117	G	A	-2.1179
118	D	A	-2.4274
119	W	A	-1.0682
120	W	A	-0.6810
121	E	A	-0.4679
122	A	A	0.0000
123	Y	A	-0.1463	mutated: RA123Y
124	S	A	0.0000
125	L	A	0.2209
126	T	A	-0.3546
127	T	A	-0.6842
128	G	A	-1.1043
129	E	A	-1.8408
130	T	A	-0.9676
131	G	A	-1.0314
132	Y	A	-0.5081
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9006
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4155
140	P	A	0.7458
141	V	A	1.7957