Project name: SH3_N136V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	NA136V
Energy difference between WT (input) and mutated protein (by FoldX)	0.300237 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.9284
• Average score: -0.8042
• Total score value: -45.8392

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9318
88	V	A	0.4412
89	A	A	0.0000
90	L	A	0.3010
91	Y	A	-0.2519
92	D	A	-2.5502
93	Y	A	-1.9206
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.3974
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0564
112	L	A	0.1429
113	N	A	-0.8875
114	S	A	-1.1778
115	S	A	-1.5952
116	E	A	-2.5582
117	G	A	-2.1301
118	D	A	-1.8866
119	W	A	-0.6355
120	W	A	-0.7262
121	E	A	-1.1521
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4992
132	Y	A	-0.8667
133	I	A	0.0000
134	P	A	0.0000
135	S	A	0.5718
136	V	A	1.9284	mutated: NA136V
137	Y	A	1.4159
138	V	A	0.0000
139	A	A	0.9918
140	P	A	0.7757
141	V	A	1.7964