Project name: SH3_L112S

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112S
Energy difference between WT (input) and mutated protein (by FoldX)	2.13474 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1025
• Maximal score value: 1.7957
• Average score: -1.0776
• Total score value: -61.4214

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4441
86	L	A	0.6290
87	F	A	0.8232
88	V	A	0.3680
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6253
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1025
99	D	A	-3.0444
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2028
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3340
108	K	A	-0.7849
109	F	A	0.0000
110	Q	A	-1.2111
111	I	A	-0.7288
112	S	A	-0.9438	mutated: LA112S
113	N	A	-1.4050
114	S	A	-1.3968
115	S	A	-1.7152
116	E	A	-2.5635
117	G	A	-2.1366
118	D	A	-2.4470
119	W	A	-1.1079
120	W	A	-1.3100
121	E	A	-1.5674
122	A	A	0.0000
123	R	A	-2.2665
124	S	A	0.0000
125	L	A	-0.2347
126	T	A	-0.6003
127	T	A	-0.9577
128	G	A	-1.6416
129	E	A	-2.3766
130	T	A	-1.9786
131	G	A	-1.6587
132	Y	A	-0.9982
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9193
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4155
140	P	A	0.7363
141	V	A	1.7957