Project name: SH3_S135T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135T
Energy difference between WT (input) and mutated protein (by FoldX)	1.97827 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9547
• Total score value: -54.4171

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5166
86	L	A	0.7923
87	F	A	0.9253
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1506
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6431
109	F	A	0.0000
110	Q	A	-0.5119
111	I	A	-0.0570
112	L	A	0.1433
113	N	A	-0.8748
114	S	A	-1.1471
115	S	A	-1.5692
116	E	A	-2.5257
117	G	A	-2.0670
118	D	A	-2.4072
119	W	A	-1.0794
120	W	A	-1.0404
121	E	A	-1.1458
122	A	A	0.0000
123	R	A	-1.7174
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6932
131	G	A	-1.5010
132	Y	A	-0.8665
133	I	A	0.0000
134	P	A	0.0000
135	T	A	-0.8949	mutated: SA135T
136	N	A	-1.1423
137	Y	A	-0.1170
138	V	A	0.0000
139	A	A	0.4191
140	P	A	0.7757
141	V	A	1.7964