Project name: wild [mutate: SA160F]

Chain sequence(s)	A: MASISSLNQIPCRTLQITSQYSKPTSKISTLPISSTNFPSKTELHRAISVKEFTYPKPKFTAQATNYDKEDEWGPEVEKISPGGVAVVDEEPPKEPSEIELLKKQLADSFYGTNRGLSASSETRAEIVELITQLESKNPNPAPTEALTLLNGKWILAYTSFSGLFPLLSRGNLLLVRVEEISQTIDSESFTVQNSVVFAGPLATTSISTNAKFEVRSPKRVQIKFEEGIIGTPQLTDSIVLPENVEFLGQKIDLSPFKGLITSVQDTASSVAKSISSQPPIKFPISNNNAQSWLLTTYLDDELRISRGDAGSVFVLIKEGSPLLKP
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	SA160F
Energy difference between WT (input) and mutated protein (by FoldX)	1.17684 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.4729
• Maximal score value: 2.4438
• Average score: -0.1084
• Total score value: -35.3265

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	0.2405
2	A	A	0.1546
3	S	A	-0.1626
4	I	A	0.1890
5	S	A	-0.2052
6	S	A	-0.2479
7	L	A	0.2591
8	N	A	-1.3835
9	Q	A	-1.2283
10	I	A	-0.0669
11	P	A	-0.2578
12	C	A	0.1970
13	R	A	-1.7223
14	T	A	-0.3657
15	L	A	-0.0225
16	Q	A	-0.0042
17	I	A	1.9282
18	T	A	0.4236
19	S	A	-0.2603
20	Q	A	-0.6892
21	Y	A	1.1248
22	S	A	0.0456
23	K	A	-0.4479
24	P	A	-0.1570
25	T	A	-0.1353
26	S	A	-0.3303
27	K	A	-1.3703
28	I	A	1.6417
29	S	A	0.1413
30	T	A	-0.0155
31	L	A	0.4914
32	P	A	0.1735
33	I	A	1.0199
34	S	A	0.1534
35	S	A	-0.1459
36	T	A	-0.0957
37	N	A	0.0000
38	F	A	0.0000
39	P	A	-0.0745
40	S	A	-0.4096
41	K	A	-1.7272
42	T	A	-0.7012
43	E	A	-1.5513
44	L	A	1.0481
45	H	A	-0.9634
46	R	A	-2.0011
47	A	A	0.0779
48	I	A	1.9843
49	S	A	0.3960
50	V	A	1.0343
51	K	A	-1.5526
52	E	A	-1.7313
53	F	A	1.6048
54	T	A	0.3207
55	Y	A	0.0000
56	P	A	-0.5285
57	K	A	-1.6549
58	P	A	-0.6835
59	K	A	-1.3663
60	F	A	1.6229
61	T	A	0.2944
62	A	A	-0.1722
63	Q	A	-1.1823
64	A	A	-0.1977
65	T	A	-0.2994
66	N	A	-1.0471
67	Y	A	0.7498
68	D	A	-1.8593
69	K	A	-2.3675
70	E	A	-2.4729
71	D	A	-2.4631
72	E	A	-1.9317
73	W	A	0.8403
74	G	A	0.0348
75	P	A	-0.3752
76	E	A	-0.5028
77	V	A	1.5024
78	E	A	-0.6484
79	K	A	-1.7636
80	I	A	-0.0251
81	S	A	-0.1631
82	P	A	-0.1159
83	G	A	-0.2343
84	G	A	-0.2024
85	V	A	0.3275
86	A	A	0.2245
87	V	A	1.1342
88	V	A	1.8291
89	D	A	-0.4910
90	E	A	-1.9582
91	E	A	-0.6269
92	P	A	-0.2199
93	P	A	-0.1589
94	K	A	-0.9184
95	E	A	-1.9657
96	P	A	-0.5648
97	S	A	0.0000
98	E	A	-1.4473
99	I	A	1.4466
100	E	A	-0.7660
101	L	A	0.4272
102	L	A	0.1580
103	K	A	-1.2807
104	K	A	-0.9597
105	Q	A	-0.2915
106	L	A	0.7718
107	A	A	0.0816
108	D	A	-0.6497
109	S	A	-0.0156
110	F	A	1.1351
111	Y	A	1.4155
112	G	A	-0.1046
113	T	A	-0.1542
114	N	A	-0.6427
115	R	A	-1.9404
116	G	A	-0.5211
117	L	A	0.1713
118	S	A	0.0187
119	A	A	0.0000
120	S	A	-0.2308
121	S	A	-0.1376
122	E	A	0.0000
123	T	A	-0.1225
124	R	A	-0.6417
125	A	A	0.0000
126	E	A	0.0000
127	I	A	0.0000
128	V	A	0.0000
129	E	A	0.0000
130	L	A	0.1861
131	I	A	0.0000
132	T	A	-0.0471
133	Q	A	-0.1083
134	L	A	0.0000
135	E	A	-0.3027
136	S	A	-0.3872
137	K	A	-1.8111
138	N	A	-0.9389
139	P	A	-0.2151
140	N	A	-0.4445
141	P	A	-0.1891
142	A	A	-0.0950
143	P	A	-0.2666
144	T	A	-0.4495
145	E	A	-1.8284
146	A	A	-0.2278
147	L	A	0.4525
148	T	A	0.1422
149	L	A	0.4230
150	L	A	0.2645
151	N	A	-0.1750
152	G	A	-0.8010
153	K	A	-1.6597
154	W	A	0.5326
155	I	A	1.4808
156	L	A	1.7449
157	A	A	0.4520
158	Y	A	0.8456
159	T	A	0.1971
160	F	A	0.3656	mutated: SA160F
161	F	A	0.3394
162	S	A	-0.0710
163	G	A	-0.1007
164	L	A	0.6862
165	F	A	1.6726
166	P	A	0.1635
167	L	A	0.9339
168	L	A	0.3616
169	S	A	-0.2282
170	R	A	-1.5665
171	G	A	-0.4070
172	N	A	0.0735
173	L	A	1.8663
174	L	A	1.3314
175	L	A	0.0000
176	V	A	1.3145
177	R	A	-1.5176
178	V	A	-0.3218
179	E	A	-1.8496
180	E	A	-0.4532
181	I	A	1.8815
182	S	A	0.0911
183	Q	A	-0.3289
184	T	A	0.0954
185	I	A	0.8023
186	D	A	-0.1312
187	S	A	-0.1509
188	E	A	0.0000
189	S	A	0.0000
190	F	A	1.9387
191	T	A	0.4240
192	V	A	0.3594
193	Q	A	-0.6432
194	N	A	-1.0516
195	S	A	0.0000
196	V	A	2.0924
197	V	A	2.4438
198	F	A	2.2641
199	A	A	0.2843
200	G	A	-0.4589
201	P	A	0.0230
202	L	A	1.5187
203	A	A	0.2309
204	T	A	-0.0803
205	T	A	-0.1225
206	S	A	0.1541
207	I	A	1.9432
208	S	A	0.1922
209	T	A	-0.3271
210	N	A	-1.2768
211	A	A	-0.4776
212	K	A	-1.3039
213	F	A	1.4560
214	E	A	-1.1547
215	V	A	1.1982
216	R	A	-1.7487
217	S	A	-0.6685
218	P	A	-0.2186
219	K	A	-2.2863
220	R	A	-1.8279
221	V	A	1.2040
222	Q	A	-0.8125
223	I	A	-0.2034
224	K	A	-1.4310
225	F	A	0.7654
226	E	A	-0.2310
227	E	A	-0.9059
228	G	A	-0.5331
229	I	A	0.0000
230	I	A	0.9902
231	G	A	0.0781
232	T	A	0.0000
233	P	A	-0.1526
234	Q	A	-0.4284
235	L	A	1.4237
236	T	A	0.0000
237	D	A	-0.4854
238	S	A	-0.0845
239	I	A	1.1903
240	V	A	2.1279
241	L	A	1.3071
242	P	A	-0.4026
243	E	A	-2.0977
244	N	A	-1.6812
245	V	A	0.0826
246	E	A	-1.7595
247	F	A	0.3806
248	L	A	1.6256
249	G	A	0.0000
250	Q	A	-1.4462
251	K	A	-1.5221
252	I	A	-0.0530
253	D	A	-0.0021
254	L	A	0.7086
255	S	A	0.0612
256	P	A	-0.0503
257	F	A	0.0000
258	K	A	-1.1239
259	G	A	0.0000
260	L	A	0.0000
261	I	A	0.0000
262	T	A	0.0000
263	S	A	0.0000
264	V	A	0.0000
265	Q	A	0.0000
266	D	A	-0.2109
267	T	A	-0.1034
268	A	A	-0.0158
269	S	A	-0.1104
270	S	A	-0.0282
271	V	A	0.1731
272	A	A	-0.1081
273	K	A	-1.0645
274	S	A	0.1094
275	I	A	1.9927
276	S	A	0.2979
277	S	A	-0.3448
278	Q	A	-0.7073
279	P	A	-0.2343
280	P	A	0.0948
281	I	A	1.6662
282	K	A	-1.1285
283	F	A	0.8439
284	P	A	0.3146
285	I	A	1.9290
286	S	A	-0.0802
287	N	A	-1.5374
288	N	A	-1.6321
289	N	A	-0.9584
290	A	A	-0.2912
291	Q	A	-1.2279
292	S	A	-0.2862
293	W	A	1.0749
294	L	A	1.7801
295	L	A	0.7279
296	T	A	0.0705
297	T	A	0.1165
298	Y	A	0.6981
299	L	A	0.0000
300	D	A	-1.0342
301	D	A	-2.0542
302	E	A	-2.0403
303	L	A	0.1300
304	R	A	0.0000
305	I	A	0.5326
306	S	A	-0.0530
307	R	A	-0.5689
308	G	A	-0.3507
309	D	A	0.0000
310	A	A	-0.0046
311	G	A	-0.0980
312	S	A	-0.0457
313	V	A	0.0000
314	F	A	0.0000
315	V	A	0.0000
316	L	A	0.1500
317	I	A	0.0000
318	K	A	-0.1077
319	E	A	0.0000
320	G	A	-0.1741
321	S	A	-0.2692
322	P	A	-0.4708
323	L	A	0.0000
324	L	A	0.3965
325	K	A	-1.3205
326	P	A	-0.5473