Chain sequence(s) |
H: EVQLVESGGGLVQPGGSLRLSCAASGFAFSTYDMSWVRQAPGKGLEWVATISSGGSYTYYLDSVKGRFTISRDSSKNTLYLQMNSLRAEDTAVYYCAPTTVVPFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTSAA L: DIQMTQSPSSLSASVGDRVTITCKASQNVRTVVAWYQQKPGKAPKTLIYLASNRHTGVPSRFSGSGSGTDFTLTISSLQPEDFATYFCLQHWSYPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Distance of aggregation | 10 Å |
Dynamic mode | No |
Mutated residues | AH228H |
Energy difference between WT (input) and mutated protein (by FoldX) | 0.898651 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
residue index | residue name | chain | Aggrescan3D score | mutation |
---|---|---|---|---|
residue index | residue name | chain | Aggrescan3D score | |
1 | D | L | -2.4047 | |
2 | I | L | 0.0000 | |
3 | Q | L | -2.0435 | |
4 | M | L | 0.0000 | |
5 | T | L | -1.1056 | |
6 | Q | L | 0.0000 | |
7 | S | L | -0.6023 | |
8 | P | L | -0.5589 | |
9 | S | L | -0.8399 | |
10 | S | L | -0.7074 | |
11 | L | L | -0.4816 | |
12 | S | L | -0.8713 | |
13 | A | L | 0.0000 | |
14 | S | L | -0.5234 | |
15 | V | L | 0.4442 | |
16 | G | L | -0.5836 | |
17 | D | L | -1.5498 | |
18 | R | L | -2.2395 | |
19 | V | L | 0.0000 | |
20 | T | L | -0.5906 | |
21 | I | L | 0.0000 | |
22 | T | L | -0.7518 | |
23 | C | L | 0.0000 | |
24 | K | L | -2.1071 | |
25 | A | L | -1.9775 | |
26 | S | L | -2.0858 | |
27 | Q | L | -2.6921 | |
28 | N | L | -2.7267 | |
29 | V | L | 0.0000 | |
30 | R | L | -2.5176 | |
31 | T | L | -1.2505 | |
32 | V | L | 0.0000 | |
33 | V | L | 0.0000 | |
34 | A | L | 0.0000 | |
35 | W | L | 0.0000 | |
36 | Y | L | 0.0000 | |
37 | Q | L | 0.0000 | |
38 | Q | L | -1.3609 | |
39 | K | L | -1.9152 | |
40 | P | L | -1.8054 | |
41 | G | L | -1.5559 | |
42 | K | L | -2.5372 | |
43 | A | L | -1.7566 | |
44 | P | L | 0.0000 | |
45 | K | L | -1.7358 | |
46 | T | L | 0.0000 | |
47 | L | L | 0.0000 | |
48 | I | L | 0.0000 | |
49 | Y | L | -0.0023 | |
50 | L | L | 0.0482 | |
51 | A | L | 0.0000 | |
52 | S | L | -0.9381 | |
53 | N | L | -1.3624 | |
54 | R | L | -2.2482 | |
55 | H | L | 0.0000 | |
56 | T | L | -1.1816 | |
57 | G | L | -1.3246 | |
58 | V | L | 0.0000 | |
59 | P | L | -0.7481 | |
60 | S | L | -0.8810 | |
61 | R | L | -1.0623 | |
62 | F | L | 0.0000 | |
63 | S | L | -0.9274 | |
64 | G | L | 0.0000 | |
65 | S | L | -0.6697 | |
66 | G | L | -1.2644 | |
67 | S | L | -1.7114 | |
68 | G | L | -2.2651 | |
69 | T | L | -2.3564 | |
70 | D | L | -2.6667 | |
71 | F | L | 0.0000 | |
72 | T | L | -0.7465 | |
73 | L | L | 0.0000 | |
74 | T | L | -0.6244 | |
75 | I | L | 0.0000 | |
76 | S | L | -1.2979 | |
77 | S | L | -1.0831 | |
78 | L | L | 0.0000 | |
79 | Q | L | -0.9910 | |
80 | P | L | -0.9771 | |
81 | E | L | -1.9898 | |
82 | D | L | 0.0000 | |
83 | F | L | -0.6380 | |
84 | A | L | 0.0000 | |
85 | T | L | -0.9062 | |
86 | Y | L | 0.0000 | |
87 | F | L | 0.0000 | |
88 | C | L | 0.0000 | |
89 | L | L | 0.0000 | |
90 | Q | L | 0.0000 | |
91 | H | L | 0.4095 | |
92 | W | L | 0.1087 | |
93 | S | L | 0.0428 | |
94 | Y | L | 0.2526 | |
95 | P | L | -0.3376 | |
96 | L | L | 0.0000 | |
97 | T | L | -0.7084 | |
98 | F | L | 0.0000 | |
99 | G | L | 0.0000 | |
100 | Q | L | -1.5518 | |
101 | G | L | 0.0000 | |
102 | T | L | 0.0000 | |
103 | K | L | -1.5679 | |
104 | V | L | 0.0000 | |
105 | E | L | 0.0000 | |
106 | I | L | -0.5692 | |
107 | K | L | -1.4288 | |
108 | R | L | -1.3012 | |
109 | T | L | -0.2490 | |
110 | V | L | 0.1517 | |
111 | A | L | -0.1236 | |
112 | A | L | -0.2554 | |
113 | P | L | 0.0000 | |
114 | S | L | -0.1716 | |
115 | V | L | 0.0000 | |
116 | F | L | 0.2573 | |
117 | I | L | 0.1100 | |
118 | F | L | 0.0000 | |
119 | P | L | -0.6987 | |
120 | P | L | 0.0000 | |
121 | S | L | -1.3418 | |
122 | D | L | -2.6345 | |
123 | E | L | -2.1510 | |
124 | Q | L | 0.0000 | |
125 | L | L | -1.8213 | |
126 | K | L | -2.5577 | |
127 | S | L | -1.5616 | |
128 | G | L | -1.3749 | |
129 | T | L | -1.1206 | |
130 | A | L | 0.0000 | |
131 | S | L | 0.0000 | |
132 | V | L | 0.0000 | |
133 | V | L | 0.0000 | |
134 | C | L | 0.0000 | |
135 | L | L | 0.0000 | |
136 | L | L | 0.0000 | |
137 | N | L | 0.0000 | |
138 | N | L | -1.3969 | |
139 | F | L | 0.0000 | |
140 | Y | L | 0.0000 | |
141 | P | L | -1.3044 | |
142 | R | L | -1.7354 | |
143 | E | L | -2.4651 | |
144 | A | L | -1.7255 | |
145 | K | L | -1.8289 | |
146 | V | L | -0.7033 | |
147 | Q | L | -0.2829 | |
148 | W | L | 0.0000 | |
149 | K | L | -0.4105 | |
150 | V | L | 0.0000 | |
151 | D | L | -1.7640 | |
152 | N | L | -1.4623 | |
153 | A | L | -0.2109 | |
154 | L | L | 0.8277 | |
155 | Q | L | -0.1991 | |
156 | S | L | -0.6272 | |
157 | G | L | -1.2545 | |
158 | N | L | -1.5707 | |
159 | S | L | -1.3236 | |
160 | Q | L | -1.1821 | |
161 | E | L | -1.1093 | |
162 | S | L | -0.5279 | |
163 | V | L | -0.3381 | |
164 | T | L | -0.9232 | |
165 | E | L | -2.0572 | |
166 | Q | L | -1.9419 | |
167 | D | L | -2.3940 | |
168 | S | L | -2.0594 | |
169 | K | L | -2.8633 | |
170 | D | L | -3.0199 | |
171 | S | L | 0.0000 | |
172 | T | L | 0.0000 | |
173 | Y | L | 0.0000 | |
174 | S | L | 0.0000 | |
175 | L | L | 0.0000 | |
176 | S | L | 0.0000 | |
177 | S | L | 0.0000 | |
178 | T | L | -0.5898 | |
179 | L | L | 0.0000 | |
180 | T | L | -0.7180 | |
181 | L | L | -0.8333 | |
182 | S | L | -1.2109 | |
183 | K | L | -2.1986 | |
184 | A | L | -1.9175 | |
185 | D | L | -2.3957 | |
186 | Y | L | 0.0000 | |
187 | E | L | -3.7177 | |
188 | K | L | -3.6147 | |
189 | H | L | -2.8936 | |
190 | K | L | -3.1515 | |
191 | V | L | -1.4821 | |
192 | Y | L | 0.0000 | |
193 | A | L | 0.0000 | |
194 | C | L | 0.0000 | |
195 | E | L | -0.7692 | |
196 | V | L | 0.0000 | |
197 | T | L | -1.0367 | |
198 | H | L | 0.0000 | |
199 | Q | L | -1.5382 | |
200 | G | L | -0.4686 | |
201 | L | L | -0.3133 | |
202 | S | L | -0.4612 | |
203 | S | L | -0.4567 | |
204 | P | L | -0.5950 | |
205 | V | L | -0.0475 | |
206 | T | L | -0.4824 | |
207 | K | L | -0.7675 | |
208 | S | L | -0.7346 | |
209 | F | L | 0.0000 | |
210 | N | L | -2.0075 | |
211 | R | L | -2.8906 | |
212 | G | L | -2.0483 | |
213 | E | L | -1.9726 | |
214 | C | L | 0.0000 | |
1 | E | H | -1.9562 | |
2 | V | H | -0.6307 | |
3 | Q | H | -1.2102 | |
4 | L | H | 0.0000 | |
5 | V | H | 1.2444 | |
6 | E | H | 0.0000 | |
7 | S | H | -0.2156 | |
8 | G | H | -0.5477 | |
9 | G | H | -0.4062 | |
10 | G | H | -0.0532 | |
11 | L | H | 0.2281 | |
12 | V | H | -0.2130 | |
13 | Q | H | -1.0693 | |
14 | P | H | -1.5002 | |
15 | G | H | -1.3446 | |
16 | G | H | -1.1320 | |
17 | S | H | -1.4511 | |
18 | L | H | -1.2710 | |
19 | R | H | -2.1016 | |
20 | L | H | 0.0000 | |
21 | S | H | -0.3026 | |
22 | C | H | 0.0000 | |
23 | A | H | 0.0109 | |
24 | A | H | 0.0000 | |
25 | S | H | -0.3568 | |
26 | G | H | -0.7201 | |
27 | F | H | 0.0000 | |
28 | A | H | -0.1996 | |
29 | F | H | 0.0000 | |
30 | S | H | -0.8209 | |
31 | T | H | -0.3237 | |
32 | Y | H | 0.0158 | |
33 | D | H | -0.2797 | |
34 | M | H | 0.0000 | |
35 | S | H | 0.0000 | |
36 | W | H | 0.0000 | |
37 | V | H | 0.0000 | |
38 | R | H | -0.6919 | |
39 | Q | H | -0.7618 | |
40 | A | H | -1.0392 | |
41 | P | H | -0.8726 | |
42 | G | H | -1.4639 | |
43 | K | H | -2.3443 | |
44 | G | H | -1.5691 | |
45 | L | H | 0.0000 | |
46 | E | H | -0.8832 | |
47 | W | H | 0.0000 | |
48 | V | H | 0.0000 | |
49 | A | H | 0.0000 | |
50 | T | H | 0.0000 | |
51 | I | H | 0.0000 | |
52 | S | H | -0.0865 | |
53 | S | H | 0.0000 | |
54 | G | H | -0.6669 | |
55 | G | H | -0.3927 | |
56 | S | H | -0.0943 | |
57 | Y | H | 1.0159 | |
58 | T | H | 0.7125 | |
59 | Y | H | 0.4138 | |
60 | Y | H | -0.6952 | |
61 | L | H | -1.3435 | |
62 | D | H | -2.6513 | |
63 | S | H | -1.6454 | |
64 | V | H | 0.0000 | |
65 | K | H | -2.7617 | |
66 | G | H | -2.0559 | |
67 | R | H | -2.3985 | |
68 | F | H | 0.0000 | |
69 | T | H | -1.0549 | |
70 | I | H | 0.0000 | |
71 | S | H | -0.2477 | |
72 | R | H | -1.1233 | |
73 | D | H | -1.3753 | |
74 | S | H | -1.3306 | |
75 | S | H | -1.4015 | |
76 | K | H | -2.2322 | |
77 | N | H | -1.5807 | |
78 | T | H | -0.8879 | |
79 | L | H | 0.0000 | |
80 | Y | H | -0.4159 | |
81 | L | H | 0.0000 | |
82 | Q | H | -1.3797 | |
83 | M | H | 0.0000 | |
84 | N | H | -2.0746 | |
85 | S | H | -1.5588 | |
86 | L | H | 0.0000 | |
87 | R | H | -2.3768 | |
88 | A | H | -1.6602 | |
89 | E | H | -2.2215 | |
90 | D | H | 0.0000 | |
91 | T | H | -0.4672 | |
92 | A | H | 0.0000 | |
93 | V | H | 0.5461 | |
94 | Y | H | 0.0000 | |
95 | Y | H | 0.0000 | |
96 | C | H | 0.0000 | |
97 | A | H | 0.0000 | |
98 | P | H | 0.0000 | |
99 | T | H | 0.0000 | |
100 | T | H | 0.7004 | |
101 | V | H | 1.3012 | |
102 | V | H | 1.1914 | |
103 | P | H | 0.1036 | |
104 | F | H | 0.5368 | |
105 | A | H | 0.0150 | |
106 | Y | H | 0.0000 | |
107 | W | H | 0.0000 | |
108 | G | H | 0.0000 | |
109 | Q | H | -1.0364 | |
110 | G | H | -0.3854 | |
111 | T | H | 0.0000 | |
112 | L | H | 1.2049 | |
113 | V | H | 0.0000 | |
114 | T | H | -0.1669 | |
115 | V | H | 0.0000 | |
116 | S | H | -0.8487 | |
117 | S | H | -0.6853 | |
118 | A | H | -0.4489 | |
119 | S | H | -0.5733 | |
120 | T | H | -0.8421 | |
121 | K | H | -1.3658 | |
122 | G | H | -1.4267 | |
123 | P | H | 0.0000 | |
124 | S | H | -0.4857 | |
125 | V | H | 0.0000 | |
126 | F | H | 0.0000 | |
127 | P | H | -1.0840 | |
128 | L | H | 0.0000 | |
129 | A | H | -0.9486 | |
130 | P | H | 0.0000 | |
131 | S | H | -1.3601 | |
132 | S | H | -1.7167 | |
133 | K | H | -2.0701 | |
134 | S | H | -1.3737 | |
135 | T | H | -0.9402 | |
136 | S | H | -0.8007 | |
137 | G | H | -0.7736 | |
138 | G | H | -0.5906 | |
139 | T | H | -0.4445 | |
140 | A | H | -0.3590 | |
141 | A | H | 0.0000 | |
142 | L | H | 0.0000 | |
143 | G | H | 0.0000 | |
144 | C | H | 0.0000 | |
145 | L | H | 0.0000 | |
146 | V | H | 0.0000 | |
147 | K | H | -0.3972 | |
148 | D | H | -0.6738 | |
149 | Y | H | 0.0000 | |
150 | F | H | 0.0000 | |
151 | P | H | -1.0088 | |
152 | E | H | -1.2531 | |
153 | P | H | -1.3239 | |
154 | V | H | 0.0000 | |
155 | T | H | -0.9596 | |
156 | V | H | -0.4674 | |
157 | S | H | -0.4049 | |
158 | W | H | 0.0000 | |
159 | N | H | -0.7670 | |
160 | S | H | -0.6392 | |
161 | G | H | -0.6351 | |
162 | A | H | -0.3127 | |
163 | L | H | -0.1027 | |
164 | T | H | -0.2385 | |
165 | S | H | -0.2207 | |
166 | G | H | -0.3359 | |
167 | V | H | 0.0823 | |
168 | H | H | -0.2966 | |
169 | T | H | 0.0149 | |
170 | F | H | 0.0000 | |
171 | P | H | -0.3973 | |
172 | A | H | -0.0910 | |
173 | V | H | 0.2075 | |
174 | L | H | 0.7291 | |
175 | Q | H | 0.1210 | |
176 | S | H | -0.1066 | |
177 | S | H | -0.2313 | |
178 | G | H | -0.0221 | |
179 | L | H | -0.2075 | |
180 | Y | H | 0.0000 | |
181 | S | H | 0.0000 | |
182 | L | H | 0.0000 | |
183 | S | H | 0.0000 | |
184 | S | H | 0.0000 | |
185 | V | H | 0.0000 | |
186 | V | H | 0.0000 | |
187 | T | H | -0.1082 | |
188 | V | H | 0.0000 | |
189 | P | H | -0.6286 | |
190 | S | H | 0.0000 | |
191 | S | H | -0.7818 | |
192 | S | H | -0.7774 | |
193 | L | H | 0.0000 | |
194 | G | H | -1.2439 | |
195 | T | H | -1.1116 | |
196 | Q | H | -1.5184 | |
197 | T | H | -1.2161 | |
198 | Y | H | 0.0000 | |
199 | I | H | -1.1642 | |
200 | C | H | 0.0000 | |
201 | N | H | -1.2853 | |
202 | V | H | 0.0000 | |
203 | N | H | -2.2736 | |
204 | H | H | 0.0000 | |
205 | K | H | -2.9654 | |
206 | P | H | -1.8080 | |
207 | S | H | -1.8799 | |
208 | N | H | -2.6231 | |
209 | T | H | -2.1637 | |
210 | K | H | -2.6518 | |
211 | V | H | -1.3936 | |
212 | D | H | -1.8890 | |
213 | K | H | -1.6422 | |
214 | K | H | -2.3522 | |
215 | V | H | 0.0000 | |
216 | E | H | -2.5723 | |
217 | P | H | -1.5522 | |
218 | K | H | -1.4699 | |
219 | S | H | -1.4187 | |
220 | C | H | -2.0202 | |
221 | D | H | -2.9692 | |
222 | K | H | -2.8133 | |
223 | T | H | -2.2217 | |
224 | H | H | -1.9255 | |
225 | T | H | -1.5091 | |
226 | S | H | -0.8050 | |
227 | A | H | -1.3669 | |
228 | H | H | -1.6851 | mutated: AH228H |