Project name: mRUBY2

Chain sequence(s)	A: LIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGHLSSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.128
• Maximal score value: 2.0386
• Average score: -0.8636
• Total score value: -186.5281

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
4	L	A	0.7555
5	I	A	0.0000
6	K	A	-2.7385
7	E	A	-2.8912
8	N	A	-3.0795
9	M	A	0.0000
10	R	A	-2.5615
11	M	A	0.0000
12	K	A	-1.5602
13	V	A	0.0000
14	V	A	-0.6076
15	M	A	0.0000
16	E	A	-2.3659
17	G	A	-2.1669
18	S	A	-2.1024
19	V	A	0.0000
20	N	A	-1.7100
21	G	A	-1.6513
22	H	A	-1.5412
23	Q	A	-2.5064
24	F	A	0.0000
25	K	A	-1.7720
26	C	A	0.0000
27	T	A	-0.6409
28	G	A	0.0000
29	E	A	-1.5921
30	G	A	0.0000
31	E	A	-2.0599
32	G	A	-1.7114
33	N	A	-1.5220
34	P	A	0.0000
35	Y	A	-0.1459
36	M	A	0.4058
37	G	A	0.0000
38	T	A	-0.7396
39	Q	A	0.0000
40	T	A	-1.4849
41	M	A	0.0000
42	R	A	-1.3466
43	I	A	0.0000
44	K	A	-0.6409
45	V	A	0.0000
46	I	A	0.2948
47	E	A	-1.8510
48	G	A	-1.4034
49	G	A	-1.1521
50	P	A	-1.0021
51	L	A	0.0000
52	P	A	-0.8094
53	F	A	0.0000
54	A	A	0.0000
55	F	A	0.0000
56	D	A	0.0000
57	I	A	0.0000
58	L	A	0.0000
59	A	A	0.0000
60	T	A	-0.0185
61	S	A	0.0000
62	F	A	0.0000
66	S	A	-0.0253
67	R	A	0.0000
68	T	A	0.0000
69	F	A	0.0000
70	I	A	0.0000
71	K	A	-0.6058
72	Y	A	-0.7254
73	P	A	-1.1074
74	K	A	-2.0690
75	G	A	-1.2719
76	I	A	0.0000
77	P	A	-1.0367
78	D	A	-0.9179
79	F	A	0.0000
80	F	A	0.0000
81	K	A	-0.1554
82	Q	A	-0.7781
83	S	A	0.0000
84	F	A	0.0000
85	P	A	-0.4359
86	E	A	-0.9183
87	G	A	0.0000
88	F	A	0.0000
89	T	A	-1.3459
90	W	A	0.0000
91	E	A	-1.8233
92	R	A	0.0000
93	V	A	0.0066
94	T	A	0.0000
95	R	A	-2.2086
96	Y	A	0.0000
97	E	A	-2.6981
98	D	A	-2.0133
99	G	A	-1.6783
100	G	A	0.0000
101	V	A	-1.2066
102	V	A	0.0000
103	T	A	-0.8617
104	V	A	0.0000
105	M	A	-0.9493
106	Q	A	0.0000
107	D	A	-1.3792
108	T	A	0.0000
109	S	A	-0.7661
110	L	A	-1.1212
111	E	A	-2.6377
112	D	A	-2.6545
113	G	A	-1.7521
114	C	A	-1.7650
115	L	A	0.0000
116	V	A	-1.0742
117	Y	A	0.0000
118	H	A	-1.0140
119	V	A	0.0000
120	Q	A	-2.2002
121	V	A	0.0000
122	R	A	-2.3714
123	G	A	0.0000
124	V	A	-1.0122
125	N	A	-1.4200
126	F	A	0.0000
127	P	A	-1.2558
128	S	A	-1.3553
129	N	A	-1.7588
130	G	A	0.0000
131	P	A	-1.9783
132	V	A	0.0000
133	M	A	-1.4153
134	Q	A	-2.2931
135	K	A	-2.4970
136	K	A	-2.8316
137	T	A	-2.4206
138	K	A	-2.7754
139	G	A	-1.8035
140	W	A	0.0000
141	E	A	-1.7706
142	P	A	-1.8028
143	N	A	-1.1592
144	T	A	-0.6429
145	E	A	0.0000
146	M	A	0.8955
147	M	A	0.0000
148	Y	A	0.7674
149	P	A	-0.2705
150	A	A	-1.3015
151	D	A	-2.3185
152	G	A	-1.7485
153	G	A	-1.7749
154	L	A	0.0000
155	R	A	-1.1573
156	G	A	0.0000
157	Y	A	1.0651
158	T	A	0.0000
159	H	A	-0.9378
160	M	A	0.0000
161	A	A	-0.8588
162	L	A	0.0000
163	K	A	-2.1695
164	V	A	-2.1359
165	D	A	-2.4993
166	G	A	-1.5688
167	G	A	-1.5540
168	G	A	-1.2453
169	H	A	-1.7434
170	L	A	0.0000
171	S	A	-1.5216
172	C	A	0.0000
173	S	A	-0.8546
174	F	A	0.0000
175	V	A	0.3035
176	T	A	0.0000
177	T	A	-1.0663
178	Y	A	0.0000
179	R	A	-2.8097
180	S	A	-1.3960
181	K	A	-1.1277
182	K	A	-0.7458
183	T	A	0.1619
184	V	A	1.0136
185	G	A	-0.2518
186	N	A	-1.2217
187	I	A	-0.9609
188	K	A	-1.6711
189	M	A	-0.7804
190	P	A	0.0000
191	G	A	0.6766
192	I	A	2.0386
193	H	A	0.0000
194	A	A	0.0000
195	V	A	0.0000
196	D	A	-1.2184
197	H	A	0.0000
198	R	A	-3.0208
199	L	A	-2.4226
200	E	A	-3.0483
201	R	A	-2.2351
202	L	A	-1.3434
203	E	A	-2.8946
204	E	A	-3.1280
205	S	A	-2.4709
206	D	A	-3.0027
207	N	A	-2.5299
208	E	A	-2.0866
209	M	A	-0.9161
210	F	A	-0.8199
211	V	A	0.0000
212	V	A	0.0000
213	Q	A	0.0000
214	R	A	-2.8109
215	E	A	-2.3572
216	H	A	-1.9535
217	A	A	0.0000
218	V	A	-0.4501
219	A	A	0.0000
220	K	A	-0.1488
221	F	A	1.6310
222	A	A	1.1409