Aggrescan3D: AB42 [mutate: HA13A, HA14A, KA16A]

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Chain sequence(s)	A: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	HA13A, HA14A, KA16A
Energy difference between WT (input) and mutated protein (by FoldX)	-0.278237 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

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The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
residue index	residue name	chain	Aggrescan3D score	mutation
1	D	A	-2.6734
2	A	A	-2.3525
3	E	A	-2.5368
4	F	A	-0.9672
5	R	A	-2.7245
6	H	A	-3.0024
7	D	A	-2.3428
8	S	A	-1.1449
9	G	A	-0.3479
10	Y	A	0.3794
11	E	A	-0.6682
12	V	A	1.1770
13	A	A	0.6735	mutated: HA13A
14	A	A	0.5967	mutated: HA14A
15	Q	A	0.8744
16	A	A	1.5236	mutated: KA16A
17	L	A	2.3123
18	V	A	1.5264
19	F	A	2.3509
20	F	A	2.3538
21	A	A	0.7043
22	E	A	-1.1749
23	D	A	-1.5045
24	V	A	-0.8129
25	G	A	-1.5906
26	S	A	-1.6770
27	N	A	-2.2191
28	K	A	-2.0655
29	G	A	-0.3976
30	A	A	0.1915
31	I	A	2.2919
32	I	A	3.1610
33	G	A	2.2906
34	L	A	3.0360
35	M	A	3.4184
36	V	A	2.9345
37	G	A	1.9898
38	G	A	1.8624
39	V	A	3.0466
40	V	A	3.8766
41	I	A	3.4983
42	A	A	1.8422